Eight New Somatic Hybrid Citrus Rootstocks with Potential for Improved Disease Resistance
Eight New Somatic Hybrid Citrus Rootstocks with Potential for Improved Disease Resistance
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DOI：
10.21273/hortsci.27.9.1033
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发表时间：
1992-09
期刊：
影响因子：
1.9
通讯作者：
E. Louzada;Jude W. Grosseti;F. Gmitter;B. Nielsen;J. L. Chandler;X. Deng;N. Tusa
中科院分区：
文献类型：
--
作者：
E. Louzada;Jude W. Grosseti;F. Gmitter;B. Nielsen;J. L. Chandler;X. Deng;N. Tusa
经聚乙二醇诱导融合后的原生质体培养，从8个互补的亲本砧木组合：柑橘(Cleopatra Mandarin)+Cleopatra aurantium L.(酸橙)、Cleopatra mandari Lush(粗柠檬)、Cleopatra mandarin(Cleopatra Mandarin)+C.volkameriana Ten获得了活力四倍体的杂种植株。&Pasq.(Volkamer Lemon)、网纹蜡梅(Cleopatra Mandarin)+柠檬蜡梅(C.limonia OSB.)(Rang-pur)、中华绒螯蟹(C.sinensis(L.)OSB。(哈姆林甜橙)+柠檬(朗普尔)，金橙(酸橙)+伏尔卡梅(Volkamer柠檬)合子苗，金合欢(光滑平坦的塞维利亚)+锦橙(粗柠檬)，中华锦鸡儿(巴伦西亚甜橙)+Carrizo柑橘(C.parisi Macf.×三叶海棠PonCirus trifoliata(L.)英国皇家空军)。从巴伦西亚甜橙和光滑平坦的塞维利亚的未融合愈伤组织的原生质体，以及酸橙、兰布尔、粗柠檬和Volkamer柠檬的未融合的叶片原生质体再生了二倍体植株。根据叶片形态、染色体数目和叶片同工酶图谱对再生植株进行分类。体细胞杂种植株均为四倍体(2n=4x=36)。一株Volkamer柠檬受精卵的同源四倍体植株被发现，显然是由同核-合子融合产生的。这8个柑橘体细胞杂交种已通过繁育和田间试验。
Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of vigorous tetraploid somatic hybrid plants from eight complemen- tary parental rootstock combinations: Citrus reticulata Blanco (Cleopatra mandarin) + C. aurantium L. (sour orange), C. reticulata (Cleopatra mandarin) + C. jambhiri Lush (rough lemon), C. reticulata (Cleopatra mandarin) + C. volkameriana Ten. & Pasq. (Volkamer lemon), C. reticulata (Cleopatra mandarin) + C. limonia Osb. (Rang- pur), C. sinensis (L.) Osb. (Hamlin sweet orange) + C. limonia (Rangpur), C. auran- tium (sour orange) + C. volkameriana (Volkamer lemon) zygotic seedling, C. auruntium hybrid (Smooth Flat Seville) + C. jambhiri (rough lemon), and C. sinensis (Valencia sweet orange) + Carrizo citrange (C. paradisi Macf. × Poncirus trifoliata (L.) Raf.). Diploid plants were regenerated from nonfused callus-derived protoplasts of Valencia sweet orange and Smooth Flat Seville and from nonfused leaf protoplasts of sour orange, Rangpur, rough lemon, and Volkamer lemon. Regenerated plants were class- ified according to leaf morphology, chromosome number, and leaf isozyme profiles. All somatic hybrid plants were tetraploid (2n = 4x = 36). One autotetraploid plant of the Volkamer lemon zygotic was recovered, apparently resulting from a homokar- yotic fusion. These eight new citrus somatic hybrids have been propagated and entered into field trials.