Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery
Continuous enzymatic hydrolysis of sugar beet pectin and L-arabinose recovery within an integrated biorefinery
复制标题
DOI：
10.1016/j.biortech.2018.08.069
复制
发表时间：
2018-12-01
影响因子：
11.4
通讯作者：
Lye, Gary J.
中科院分区：
文献类型：
--
作者：
Cardenas-Fernandez, Max;Hamley-Bennett, Charlotte;Lye, Gary J.
甜菜浆（SBP）通过蒸汽爆破分级，释放甜菜果胶（SB-果胶），其使用新型α-L-阿拉伯呋喃糖苷酶（AF）选择性水解，产生单体L-阿拉伯糖（Ara）和富含半乳糖醛酸的主链（GABB）。AF固定在环氧官能化树脂上，总固定产率为70%。SB-果胶的预处理，以去除有色化合物，提高了固定化AF的稳定性，允许其在搅拌釜反应器中重复使用多达10个反应循环。SB-果胶的连续水解，随后使用固定化AF填充床反应器（PBR）进行。反应器的性能进行了评价，使用实验设计的方法。预处理的SB-果胶水解连续进行7天，保持73%的PBR性能。采用切向流超滤技术实现了阿糖胞苷与GABB的连续分离，阿糖胞苷的回收率为92%。这些结果表明，建立一个连续的生物过程，以获得阿糖胞苷从廉价的SBP生物质的可行性。
Sugar beet pulp (SBP) fractionated by steam explosion, released sugar beet pectin (SB-pectin) which was selectively hydrolysed using a novel alpha-L-arabinofuranosidase (AF), yielding monomeric L-arabinose (Ara) and a galacturonic acid rich backbone (GABB). AF was immobilised on an epoxy-functionalised resin with 70% overall immobilisation yield. Pretreatment of SB-pectin, to remove coloured compounds, improved the stability of the immobilised AF, allowing its reutilisation for up to 10 reaction cycles in a stirred tank reactor. Continuous hydrolysis of SB-pectin was subsequently performed using a packed bed reactor (PBR) with immobilised AF. Reactor performance was evaluated using a Design of Experiment approach. Pretreated SB-pectin hydrolysis was run for 7 consecutive days maintaining 73% of PBR performance. Continuous separation of Ara from GABB was achieved by tangential flow ultrafiltration with 92% Ara recovery. These results demonstrate the feasibility of establishing a continuous bioprocess to obtain Ara from the inexpensive SBP biomass.