In vitro response of MC3T3-E1 preosteoblasts within three-dimensional apatite-coated PLGA scaffolds
In vitro response of MC3T3-E1 preosteoblasts within three-dimensional apatite-coated PLGA scaffolds
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DOI：
10.1002/jbm.b.30261
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发表时间：
2005-10-01
影响因子：
3.4
通讯作者：
Wu, BM
中科院分区：
文献类型：
--
作者：
Chou, YF;Dunn, JCY;Wu, BM
仿生磷灰石已被报道在许多体内和体外模型中促进成骨活性，但磷灰石微环境促进这种活性的确切机制尚不清楚。这种机制的研究需要可重复的模型系统，是相关的组织工程实践。尽管二维（2D）磷灰石涂覆的聚苯乙烯培养皿提供了实用性和再现性，但它们不能模拟三维（D）微环境和降解聚合物基质的效果。本文报道了一个简单的三维模型系统来解决这些相关的影响，并利用其在体外磷灰石促进成骨细胞分化的调查。通过将聚乳酸-共-乙交酯（PLGA）支架依次浸入不同的模拟体液（SBF）中实现磷灰石涂层。SEM、EDX、FTIR、TEM电子衍射证实磷灰石涂层由缺钙碳酸化羟基磷灰石晶体组成。虽然磷灰石涂层和非涂层PLGA支架支持MC 3 T3-E1的附着、扩散和增殖，但观察到成骨细胞分化的显著差异。相对于未包被的对照，定量实时PCR显示碱性磷酸酶（ALP）的显著磷灰石相关抑制，骨桥蛋白（OPN）在3天的早期上调，骨钙素（OCN）和骨唾液蛋白（BSP）在4周的上调。总之，在与组织工程相关的3D模型系统中可以观察到磷灰石促进的成骨细胞分化。(c)2005年，Wiley期刊。Inc.
Biomimetic apatites have been reported to promote osteogenic activities in numerous in vivo and in vitro models, but the precise mechanism by which the apatite micro-environment promotes such activities is not well understood. Such mechanistic studies require reproducible model systems that are relevant to tissue engineering practices. Although two-dimensional (2D) apatite-coated polystyrene culture dishes provide practicality and reproducibility, they do not simulate the effects of the three-dimensional (D) microenvironment and degrading polymeric substrates. A simple 3D model system to address these relevant effects, and its utilization in the investigation of apatite-promoted osteoblastic differentiation in vitro is reported in this paper. Apatite coating was achieved by sequentially immersing poly(lactide-co-glycolide) (PLGA) scaffolds into different simulated body fluids (SBF). SEM, EDX, FTIR, TEM electron diffraction confirmed the apatite coating to comprise of calcium-deficient carbonated hydroxyapatite crystals. While both apatite-coated and noncoated PLGA scaffolds supported MC3T3-E1 attachment, spreading, and proliferation, significant differences in osteoblastic differentiation were observed. Relative to noncoated controls, quantitative real-time PCR revealed significant apatite-associated suppression of alkaline phosphatase (ALP), early upregulation of osteopontin (OPN) at 3 days, and upregulation of osteocalcin (OCN) and bone sialoprotein (BSP) at 4 weeks. In summary, apatite-promoted osteoblastic differentiation can be observed in a 3D model system that is relevant to tissue engineering. (c) 2005 Wiley Periodicals. Inc.