Endowing a ferritin-like cage protein with high affinity and selectivity for certain inorganic materials
Endowing a ferritin-like cage protein with high affinity and selectivity for certain inorganic materials
复制标题
DOI：
10.1002/smll.200500010
复制
发表时间：
2005-08-01
期刊：
影响因子：
13.3
通讯作者：
Shiba, K
中科院分区：
文献类型：
--
作者：
Sano, K;Ajima, K;Shiba, K
铁蛋白是一种笼状蛋白质，具有纳米尺寸的内部空间（直径7-8 nm），[1]其中各种无机纳米颗粒包括氧化的Fe，[2]氧化的Co，[3]氧化的U，[4] CdSe，[5]和CdS [6]可以生物矿化。这种含有纳米颗粒的铁蛋白是一种很有前途的纳米纤维素的构建块;然而，铁蛋白在无机基质上的位点选择性组装的方法尚未建立。我们设计了马铁蛋白L链的基因，以便在其N末端附加一个短肽适体（针对钛[7]或碳纳米角[8]）。重组的铁蛋白样笼蛋白
Ferritin is a cage-shaped protein with a nanometer-sized interior space (7–8 nm in diameter),[1] in which a variety of inorganic nanoparticles including oxidized Fe,[2] oxidized Co,[3] oxidized U,[4] CdSe,[5] and CdS [6] can be biomineralized. This nanoparticle-containing ferritin is a promising building block for nanofabrication; however, the methods for siteselective assembly of ferritin on an inorganic matrix are not yet established. We engineered the gene for the L chain of horse ferritin so that a short peptide aptamer (against titanium [7] or carbon nanohorns [8]) would be appended at its N-terminal. The reconstituted ferritin-like cage proteins re-