STABILITY OF AMORPHOUS STATE IN SYSTEM WATER-GLYCEROL-DIMETHYLSULFOXIDE
STABILITY OF AMORPHOUS STATE IN SYSTEM WATER-GLYCEROL-DIMETHYLSULFOXIDE
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DOI：
10.1016/0011-2240(78)90012-3
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发表时间：
1978-01-01
期刊：
影响因子：
2.7
通讯作者：
KAUFMANN, A
中科院分区：
文献类型：
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作者：
BOUTRON, P;KAUFMANN, A
在同时含有甘油和二甲基亚砜的水溶液中，用X射线衍射法确定了淬火后复温过程中的各种状态。在很低的温度下观察到了整个溶液的无定形状态。只有在主要含有DMSO的溶液中，才能在复温后的X射线中看到共晶。在其他溶液中，只看到了纯冰。如果存在足够的DMSO，它直接在六角形系统中结晶；否则，立方冰和六角冰的混合物首先出现。在较宽的升温速率范围内，用差示扫描量热仪测量了熔融结束温度和析晶温度。从这些测量中可以推断出非晶态的稳定性，其定义是不发生晶化的临界升温速率。这种稳定性没有出现最大值，但在给定的水浓度下，从甘油到DMSO的稳定性增加。在DMSO溶液中加入少量甘油，可大大提高共晶结晶的难度，而不会大大降低整个溶液的非晶态稳定性。那些含有约10%(重量/重量)甘油/(甘油+DMSO)的低温保护剂比那些只含有DMSO的低温保护剂更好，至少对于低冷却或低升温速率，在这种情况下，共晶可能有足够的时间结晶，最终在细胞外或细胞内产生有害影响。
In aqueous solutions containing both glycerol and DMSO [dimethylsulfoxide], the various states during rewarming after quenching were identified by X-ray diffraction. The amorphous state of the whole solution was observed at very low temperatures. The eutectic was seen by X-rays after rewarming only in the solutions containing mainly DMSO. In the other solutions only pure ice was seen. It crystallizes directly in the hexagonal system, if enough DMSO is present; otherwise, a mixture of cubic and hexagonal ice appears first. The temperature of the end of fusion and the devitrification temperature were measured with a scanning differential calorimeter for a wide range of warming rates. From these measurements was deduced the stability of the amorphous state, defined by the critical heating rate above which no crystallization occurs. That stability presents no maximum, but increases from glycerol to DMSO for a given water concentration. A small amount of glycerol in a solution of DMSO greatly enhances the difficulty of crystallization of the eutectic, without decreasing the stability of the amorphous state of the whole solution by much. Those containing about 10% (weight/weight) glycerol/(glycerol + DMSO) are better cryoprotectants than those with only DMSO, at least for low cooling or warming rates where the eutectic may have enough time to crystallize, eventually with deleterious effects, outside or inside the cells.