Factors Influencing Cryopreservation of Domestic Animal Embryos
Factors Influencing Cryopreservation of Domestic Animal Embryos
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影响家畜胚胎冷冻保存的因素
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
S. Leibo
中科院分区：
文献类型：
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作者：
A. Massip;S. Leibo
将胚胎移植到合适的受体后，对胚胎发育潜力的最终考验是正常幼崽的出生。然而，胚胎在冷冻压力下存活的能力可以被认为是最初的“筛选测试”。通常根据其形态选择体内衍生的胚胎。一般来说，根据形态学确定的胚胎“质量”与妊娠可能性之间存在良好的关联（如果不是相关性的话）（Wright，1985）。这种对应关系甚至适用于冷冻保存后的胚胎（Niemann et al.，1982; Kennedy等人，1983; Leibo，1986）。例如，虽然给胚胎分配“等级”是一个相当主观的判断，但可以根据胚胎的形态给胚胎分配数字等级。图1所示的妊娠结果表明，妊娠的可能性可以从分配给它的胚胎的形态学等级来估计，无论是作为新鲜胚胎，还是冷冻前的冷冻保存胚胎。一般来说，质量较差的胚胎不太可能怀孕，而质量较好的胚胎更有可能怀孕。这些结果还表明，对于给定等级的胚胎，冷冻确实会降低怀孕的可能性。一些被归类为“好”或“优秀”的胚胎如果作为新鲜胚胎转移可能会导致怀孕，但如果在冷冻后转移则不会。具有相同形态的胚胎不一定具有相同的怀孕可能性。
The ultimate test of the developmental potential of an embryo, after its transfer into a suitable recipient, is the birth of a normal young animal. Yet, the ability of an embryo to survive the stresses of freezing can be considered as an initial “screening test”. Embryos that have been derived in vivo are usually selected on the basis of their morphology. In general, there appears to be a good association, if not a correlation, between embryo “quality”, assigned on the basis of morphology, and the likelihood of pregnancy (Wright, 1985). This correspondence even holds for embryos after they have been cryopreserved (Niemann et al., 1982; Kennedy et al., 1983; Leibo, 1986). For example, although assigning a “grade” to an embryo is a rather subjective judgement, embryos can be assigned numerical grades on the basis of their morphology. The pregnancy results shown in Figure 1 illustrate that the likelihood of pregnancy can be estimated from the morphological grade of an embryo assigned to it either as a fresh embryo, or for a cryopreserved embryo prior to its being frozen. In general, poorer quality embryos are less likely to yield a pregnancy, and better quality embryos are more likely. Those results also indicate that, for a embryo of a given grade, freezing does reduce the likelihood of pregnancy. Some embryos classified as “good” or “excellent” may result in pregnancy if transferred as fresh embryos, but not if transferred after having been frozen. Embryos with identical morphologies do not necessarily have the same likelihood of producing a pregnancy.