DOES MYELOMA SECRETE AN OSTEOBLAST INHIBITING FACTOR
DOES MYELOMA SECRETE AN OSTEOBLAST INHIBITING FACTOR
复制标题
DOI：
10.1302/0301-620x.71b2.2925748
复制
发表时间：
1989-03-01
影响因子：
--
通讯作者：
WARD, C
中科院分区：
文献类型：
--
作者：
EVANS, CE;GALASKO, CSB;WARD, C
与大多数其他肿瘤不同，骨髓瘤导致骨破坏而不产生成骨反应；我们试图评估骨髓瘤是否分泌一种抑制成骨细胞的体液因子。人骨源性细胞与骨髓瘤细胞共培养，或在骨髓瘤细胞条件培养液中培养。用细胞计数和~3H-TdR掺入法检测骨源性细胞的生长，骨髓瘤细胞条件培养液对骨源性细胞生长有抑制作用，骨髓瘤细胞与骨髓瘤细胞共培养对骨源性细胞生长有一定的抑制作用。抑制作用呈剂量依赖关系，也依赖于调节培养液的骨髓瘤细胞的密度。我们的研究结果表明，骨髓瘤分泌一种小于50,000道尔顿分子量的成骨细胞抑制因子。
Unlike most other tumours, myeloma causes bone destruction without an osteoblastic reaction; we tried to assess whether myeloma secretes a humoral factor that inhibits osteoblasts. Human bone-derived cells were either co-cultured with myeloma cells, or cultured in medium conditioned by myeloma cells. Bone-derived cell growth was measured by cell counts and by uptake of tritiated thymidine (3H-Tdr); growth was inhibited when cultured in medium conditioned by myeloma cells and some inhibition was seen when the bone-derived cells were co-cultured with myeloma cells. The inhibiting effect was dose-dependent and also dependent upon the density of the myeloma cells conditioning the medium. The results of our study suggest that myeloma secretes an osteoblast inhibiting factor of less than 50,000 Dalton molecular weight.