CHEMICAL AND BIOLOGICAL EVIDENCE LINKS MICROCYSTINS TO SALMON NETPEN LIVER-DISEASE
CHEMICAL AND BIOLOGICAL EVIDENCE LINKS MICROCYSTINS TO SALMON NETPEN LIVER-DISEASE
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DOI：
10.1016/0041-0101(93)90404-7
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发表时间：
1993-10-01
期刊：
影响因子：
2.8
通讯作者：
WILLIAMS, DE
中科院分区：
文献类型：
--
作者：
ANDERSEN, RJ;LUU, HA;WILLIAMS, DE
有证据表明，微囊藻毒素与一种严重的肝病有关，这种疾病发生在不列颠哥伦比亚省沿海网养的大西洋鲑鱼中。对患有网状肝病的大西洋鲑鱼的肝组织提取物进行的高效液相连接蛋白磷酸酶生物分析表明，存在一种蛋白磷酸酶抑制剂，在层析上与微囊藻毒素-LR难以区分。对健康对照鱼肝组织的分析显示，微囊藻毒素-LR完全缺失。将微囊藻毒素-LR注入健康大西洋鲑鱼体内，可重现网状肝病的病理改变，包括弥漫性坏死和肝巨细胞增多症。
Evidence is presented that links microcystins to a severe liver disease that occurs in Atlantic salmon that are netpen-reared in coastal British Columbia. Liquid chromatography-linked protein phosphatase bioassay analysis of extracts of liver tissue taken from Atlantic salmon afflicted with netpen liver disease showed the presence of an inhibitor of protein phosphatase that was chromatographically indistinguishable from microcystin-LR. Analysis of liver tissue from healthy control fish showed a complete absence of microcystin-LR. Intraperitoneal injection of microcystin-LR into healthy Atlantic salmon re-created the pathologic changes of netpen liver disease, including diffuse necrosis and hepatic megalocytosis.