ELECTRON-MICROSCOPIC EXAMINATION OF WASTEWATER BIOFILM FORMATION AND STRUCTURAL COMPONENTS
ELECTRON-MICROSCOPIC EXAMINATION OF WASTEWATER BIOFILM FORMATION AND STRUCTURAL COMPONENTS
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DOI：
10.1128/aem.45.6.1921-1931.1983
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发表时间：
1983-01-01
影响因子：
4.4
通讯作者：
BISHOP, PL
中科院分区：
文献类型：
--
作者：
EIGHMY, TT;MARATEA, D;BISHOP, PL
本研究记录了原位废水生物膜的形成，结构和物理化学性质所揭示的扫描和透射EM。阳离子化铁蛋白用于标记生物膜糖萼的阴离子位点以在薄切片中观察。废水生物膜的形成是海洋生物膜形成的过程。扫描EM显示在144小时内细胞定殖和生长的急剧增加。成分包括各种活跃的分裂形态类型。许多定居的细菌都有鞭毛。在表面的初级定殖后观察到丝状体。透射电镜显示占主导地位的革兰氏阴性细胞壁结构的生物膜成分。至少观察到3种类型的糖萼。占优势的糖萼具有间隙和密集的阳离子化铁蛋白标记。两个糖萼似乎介导生物膜粘附到基质。因此，这种薄的废水生物膜的主要糖萼显然部分地用于将细菌封闭在基质中并将生物膜锚在基质上并提供具有聚阴离子性质的广泛表面积。
This research documents in situ wastewater biofilm formation, structure and physicochemical properties as revealed by scanning and transmission EM. Cationized ferritin was used to label anionic sites of the biofilm glycocalyx for viewing in thin section. Wastewater biofilm formation paralleled the processes involved in marine biofilm formation. Scanning EM revealed a dramatic increase in cell colonization and growth over a 144 h period. Constituents included a variety of actively dividing morphological types. Many of the colonizing bacteria were flagellated. Filaments were seen after primary colonization of the surface. Transmission EM revealed a dominant gram-negative cell wall structure in the biofilm constituents. At least 3 types of glycocalyces were observed. The predominant glycocalyx possessed interstices and was densely labeled with cationized ferritin. Two of the glycocalyces appeared to mediate biofilm adhesions to the substratum. Thus, the predominant glycocalyx of this thin wastewater biofilm evidently serves, in part, to enclose the bacteria in a matrix and anchor the biofilm to the substratum and to provide an extensive surface area with polyanionic properties.