Gradient formation of the TGF-β homolog Dpp
Gradient formation of the TGF-β homolog Dpp
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DOI：
10.1016/s0092-8674(00)00200-2
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发表时间：
2000-12-08
期刊：
影响因子：
64.5
通讯作者：
González-Gaitán, M
中科院分区：
文献类型：
--
作者：
Entchev, EV;Schwabedissen, A;González-Gaitán, M
分泌的形态发生素，如果蝇TGF-β同源物Decapentaplegic（Dpp），被认为通过靶组织扩散，并形成提供位置信息的长程浓度梯度。使用GFP-Dpp融合，我们监测了TGF-β家族成员在整个靶组织中原位运输并形成长程浓度梯度。证据表明，长距离Dpp运动涉及Dpp受体和发动蛋白的功能。我们还表明，内吞运输和降解的速率决定Dpp信号范围。我们提出了一个模型，其中梯度是通过细胞内运输形成的受体介导的内吞作用的配体在接收细胞与梯度斜率控制的内吞分选DPP回收与降解。
Secreted morphogens such as the Drosophila TGF-beta homolog Decapentaplegic (Dpp) are thought to spread through target tissues and form long-range concentration gradients providing positional information. Using a GFP-Dpp fusion, we monitored a TGF-beta family member trafficking in situ throughout the target tissue and forming a long-range concentration gradient. Evidence is presented that long-range Dpp movement involves Dpp receptor and Dynamin functions. We also show that the rates of endocytic trafficking and degradation determine Dpp signaling range. We propose a model where the gradient is formed via intracellular trafficking initiated by receptor-mediated endocytosis of the ligand in receiving cells with the gradient slope controlled by endocytic sorting of Dpp toward recycling versus degradation.