Rice pollen aperture formation is regulated by the interplay between OsINP1 and OsDAF1.
Rice pollen aperture formation is regulated by the interplay between OsINP1 and OsDAF1.
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水稻花粉孔径的形成受 OsINP1 和 OsDAF1 之间的相互作用调节。
DOI：
10.1038/s41477-020-0630-6
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Liang Wanqi
中科院分区：
文献类型：
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作者：
Zhang Xu;Zhao Guochao;Tan Qian;Yuan Hui;Betts Natalie;Zhu Lu;Zhang Dabing;Liang Wanqi
花粉表面的孔为出现的花粉管提供出口。在不同的植物物种中，气孔表现出巨大的形态差异——包括水稻在内的禾草，具有一个由环和盖组成的复杂的气孔——但人们对这种物种特异性的细胞表面模式是如何形成的知之甚少。在这里，我们报道了一种在水稻中发现的凝集素受体样激酶OsDAF1，它对环的形成和繁殖至关重要。OsDAF1在早期小孢子细胞中分布均匀，但在四分体阶段定位于远端孔前部位。我们进一步揭示了拟南芥中一个关键的孔径因子OsINP1的水稻同源物在水稻孔径形成中具有保守和多样化的作用。OsINP1的破坏阻止了孔洞的形成，阻碍了花粉管的萌发。此外，我们的研究结果表明，OsINP1是OsDAF1通过直接蛋白相互作用极化所必需的，这表明OsINP1在拟南芥中缺失的环的形成中具有额外的作用。我们的研究揭示了水稻花粉孔对产量的重要性，揭示了谷类植物花粉孔发育的控制机制。
The aperture on the pollen surface provides an exit for the emerging pollen tube. Apertures exhibit huge morphological variation across plant species—grasses, including rice, possess a complex aperture consisting of an annulus and an operculum—but little is known about how this species-specific cell-surface pattern forms. Here, we report a lectin receptor-like kinase inOryza sativa, OsDAF1, which is essential for annulus formation and thus for fertility. OsDAF1 is evenly distributed in early microsporocytes but localizes to the distal pre-aperture site at the tetrad stage. We further reveal that the rice orthologue of a key aperture factor inArabidopsis, OsINP1, has conserved and diversified roles in rice aperture formation. Disruption of OsINP1 prevents formation of the aperture, precluding pollen-tube germination. Furthermore, our results demonstrate that OsINP1 is required for polarization of OsDAF1 via direct protein interaction, suggesting that OsINP1 has an additional role in the formation of annulus that is absent inArabidopsis. Our study reveals the importance of the aperture for rice grain yield and reveals mechanisms controlling pollen aperture development in cereal species.