Chronic treatment with propranolol induces antioxidant changes and protects against ischemia-reperfusion injury
Chronic treatment with propranolol induces antioxidant changes and protects against ischemia-reperfusion injury
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使用普萘洛尔进行慢性治疗可诱导抗氧化剂变化并防止缺血再灌注损伤
DOI：
10.1006/jmcc.1997.0558
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发表时间：
1997-12-01
影响因子：
5
通讯作者：
Singal, PK
中科院分区：
文献类型：
--
作者：
Khaper, N;Rigatto, C;Singal, PK
本研究的目的是研究慢性心得安是否对缺血再灌注损伤有保护作用，是否引起心肌内源性抗氧化酶活性及其基因表达的变化。大鼠分别给予心得安（10 mg/kg/天，i.p） 6天或18天，最后一次心得安注射后48天，离体心脏进行60 min的全身缺血和40 min的再灌注。对照组和治疗组缺血后静息张力分别为385 +/- 30和150 +/- 15%；和再灌注是140 + / - 11和49 + / - 6%,分别比pre-ischemic值,恢复全球缺血性心脏收缩功能的再灌注后约35%在治疗组在对照组相比约16%在10到20分钟,积极应对儿茶酚胺在心中从普萘洛尔组(C, 3.41 + / - 0.36, epi 6.03 + / - 0.47 g / g),并与控制心(C, 3.55 + / - 0.31;心肌抗氧化剂、过氧化氢酶和谷胱甘肽过氧化物酶活性在缺血再灌注前分别比对照组提高了67 +/- 9%和45 +/- 11%，超氧化物歧化酶活性未见变化。三种抗氧化酶的mRNA表达在治疗组心脏中与对照组相比没有变化，在缺血-再灌注发作前后，心得安处理的心脏中脂质过氧化与对照组相比均显著降低，再灌注结束时研究的心脏中酶活性在治疗组与对照组之间没有差异。这些数据表明，在缺乏β -阻断剂的情况下，心得安治疗动物对离体心脏缺血再灌注损伤有保护作用。心得安治疗增加内源性抗氧化酶活性可能在这种保护中起作用。(C) 1997学术出版社有限公司
The goal of this study was to examine whether chronic administration of propranolol offers protection against ischemia-reperfusion injury and whether it induces any change in the myocardial endogenous antioxidant enzyme activities and their gene expression, Rats were treated with propranolol (10 mg/kg/day, i.p.) for either 6 or 18 days, Forty-eighth after the last propranolol injection, isolated hearts were subjected to 60 min of global ischemia and 40 min of reperfusion, Resting tension in the control and treated groups after ischemia was 385 +/- 30 and 150 +/- 15%; and upon reperfusion was 140 +/- 11 and 49 +/- 6%, respectively as compared to the pre-ischemic values, Recovery of the contractile function in globally ischemic hearts upon reperfusion was about 35% in the treated group as compared to about 16% in the control group at 10 and 20 min, A positive response to catecholamine was observed in hearts from propranolol group (C, 3.41 +/- 0.36; epi, 6.03 +/- 0.47 g/g) and was comparable to control hearts (C, 3.55 +/- 0.31; epi, 6.48 +/- 0.42 g/g), Myocardial antioxidants, catalase and glutathione peroxidase enzyme activities, in the treated group, prior to ischemia-reperfusion were increased by 67 +/- 9 and 45 +/- 11%, respectively, over those in controls, Superoxide dismutase activity did not show any change. The mRNA expression for the three antioxidant enzymes did not change in the hearts of the treated group as compared to control, Lipid peroxidation, both before and after the ischemia-reperfusion episode, was significantly reduced in the propranolol-treated hearts compared to the control group, Hearts studied at the end of reperfusion showed no difference in enzyme activities between treated and control groups, These data show that propranolol treatment of the animals protects against ischemia-reperfusion injury in isolated hearts in the absence of beta-blockade. Increased endogenous antioxidant enzyme activities due to propranolol treatment may have a role in this protection. (C) 1997 Academic Press Limited.