Solid State 19F-NMR Analysis of Oriented Biomembranes
Solid State 19F-NMR Analysis of Oriented Biomembranes
复制标题
定向生物膜的固态 19F-NMR 分析
DOI：
10.1007/1-4020-3910-7_31
复制
发表时间：
2008
影响因子：
2.1
通讯作者：
A. Ulrich
中科院分区：
文献类型：
--
作者：
A. Ulrich
19 F核为固态NMR提供了许多优点，首先是它的高灵敏度，只有1H超过。特别是在不可结晶的生物样品中，例如膜和纤维，缺乏天然丰度背景通常使选择性19 F标记成为首选方法[1-3]。各向异性的相互作用是可访问的宏观取向的样品，其光谱线型很容易解释的强大的1脉冲实验。因此，这种策略对于取向膜和取向纤维特别有用。魔角旋转实验在这里不包括，虽然他们当然也是一个丰富的信息来源[4-7]。在下文中，我们将描述合成肽中的一个或几个氟标记如何提供有关其局部对齐、其动态行为及其在脂质膜中的三维结构的信息。关于19 F-NMR结构分析的方法学方面和膜活性肽的生物学应用的进一步细节在最近的综述中被覆盖[8-11]。
The19F nucleus offers numerous advantages for solid state NMR, being first and foremost its high sensitivity that is only exceeded by1H. Especially in non-crystallizable biological samples, such as membranes and fibers, the lack of a natural abundance background often makes selective19F-labeling the method of choice [1–3]. Anisotropie interactions are accessible in robust 1-pulse experiments on macroscopically oriented samples, whose spectral lineshapes are readily interpreted. This strategy is thus particularly useful for oriented membranes and aligned fibers. Magic angle spinning experiments are not covered here, though they are of course also a fruitful source of information [4–7]. In the following, we will describe how one or a few fluorine labels in a synthetic peptide can provide information about its local alignment, its dynamic behavior, and its three-dimensional architecture in a lipid membrane. Further details about the methodological aspects of19F-NMR structure analysis and biological applications to membrane-active peptides are covered in recent reviews [8–11].