Aneuploidy of chromosome 8 detected by fluorescence in situ hybridisation in ACPO1 cell line gastric adenocarcinoma
Aneuploidy of chromosome 8 detected by fluorescence in situ hybridisation in ACPO1 cell line gastric adenocarcinoma
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DOI：
10.1007/s10238-006-0108-5
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发表时间：
2006-10-01
影响因子：
4.6
通讯作者：
Burbano, R. Rodriguez
中科院分区：
文献类型：
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作者：
Guimaraes, A. Costa;Quintana, L. Goncalves;Burbano, R. Rodriguez
胃癌是世界上第三大最常见的肿瘤类型和第二大死亡原因。ACP 01是巴西开发的第一个胃腺癌细胞系。为了评估胃癌发生中涉及的染色体畸变，我们分析了三个不同代次（第6，12和35）的ACP 01细胞系的荧光原位杂交使用染色体8 α-卫星探针。发现的大多数8号染色体改变涉及该染色体的数量增加。在所有病例中均检测到8号染色体三体，范围为37%（第6代）至67%（第35代），8号染色体四体（也在所有传代中观察到）范围为2.5%（第6代）至30%（第35代）。在所有传代中观察到8号染色体存在5个信号，在第12代中发现频率最高（20%）。我们的结果证实8号染色体三体是胃腺癌的一种常见生物学现象，并可作为胃粘膜恶性肿瘤的标志物。虽然胃肿瘤是常见的肿瘤，但在文献中关于其细胞遗传学的论文很少。因此，有必要进行新的研究，旨在确定肿瘤的特殊遗传特征，这可能有助于这种疾病的诊断和预后，此外还可以建立更准确的治疗方法。
Gastric cancer is the third most frequent type of neoplasia and the second most important cause of death in the world. ACP01 is the first gastric adenocarcinoma cell line developed in Brazil. To evaluate chromosomal aberrations implicated in gastric carcinogenesis, we analysed three different passages (6th, 12th and 35th) of ACP01 cell line by fluorescence in situ hybridisation using chromosome 8 alpha-satellite probe. Most of the chromosome 8 alterations found involved a numerical increase of this chromosome. Chromosome 8 trisomy was detected in all cases, varying from 37% (6th passage) to 67% (35th passage), and chromosome 8 tetrasomy (also observed in all passages) varied from 2.5% (6th passage) to 30% (35th passage). The presence of five signals for chromosome 8 was observed in all passages with the highest frequency found in the 12th passage (20%). Our results confirm that trisomy of chromosome 8 is a common biological phenomenon in adenocarcinoma of stomach and can be used as a gastric mucosa malignancy marker. Although gastric tumours are frequent neoplasias, papers on their cytogenetics are scarce in the literature. It is, therefore, necessary to conduct new studies aiming to identify peculiar genetic characteristics of a tumour, which might help in diagnosis and prognosis of this disease, besides allowing more accurate therapeutic conduct to be established.