Feasibility study on surface morphology regulation of β-tricalcium phosphate bone graft for enhancing cellular response
Feasibility study on surface morphology regulation of β-tricalcium phosphate bone graft for enhancing cellular response
复制标题
β-磷酸三钙骨移植物表面形态调控增强细胞反应的可行性研究
DOI：
10.1016/j.ceramint.2022.02.200
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发表时间：
2022
影响因子：
5.2
通讯作者：
Ishikawa Kunio
中科院分区：
文献类型：
--
作者：
Putri Tansza Setiana;Sunarso;Hayashi Koichiro;Tsuru Kanji;Ishikawa Kunio
β-磷酸三钙（βTCP）颗粒通常用作人工骨移植材料。同时，骨移植物的表面形态是影响细胞反应的重要因素。本研究探讨了βTCP骨移植物表面形态调控对增强细胞反应的可行性。该调节是在多步加热过程的基础上实现的。简而言之，βTCP颗粒在1300 °C下初始热处理12小时导致具有互连微孔的α-磷酸三钙（αTCP）颗粒的制造。在第二次热处理中，将αTCP颗粒暴露于100 °C下的100%相对湿度导致αTCP部分水解，导致在αTCP表面上制造针状缺钙羟基磷灰石（cdHAp）晶体。在1100 °C的第三次热处理后，cdHAp和αTCP都转化回βTCP，并形成具有粗糙表面结构的相互连接的微孔。体外细胞评价表明，与未处理的βTCP颗粒相比，通过一系列热处理获得的βTCP颗粒在第9天的细胞增殖率提高了24倍，碱性磷酸酶活性提高了4倍。因此，我们得出结论，通过一系列热处理调节表面形态有助于改善βTCP骨移植材料的细胞反应。
Beta-tricalcium phosphate (βTCP) granules are commonly used as an artificial bone graft material. Meanwhile, the surface morphology of the bone graft is an important factor for cellular response. In this study, feasibility study on surface morphology regulation of βTCP bone graft for enhancing cellular response were investigated. The regulation was achieved on the basis of a multistep heating process. Briefly, initial heat treatment of βTCP granules at 1300 °C for 12 h resulted in fabrication of alpha-tricalcium phosphate (αTCP) granules with interconnected micropores. In the second heat treatment, exposure of αTCP granules to 100% relative humidity at 100 °C resulted in partial hydrolysis of αTCP, leading to the fabrication of needle-like calcium-deficient hydroxyapatite (cdHAp) crystals on the αTCP surface. After the third heat treatment at 1100 °C, both cdHAp and αTCP converted back to βTCP, and interconnected micropores with a roughened surface structure were formed.In vitrocell evaluation demonstrated that βTCP granules obtained by the series of heat treatments exhibited 24 times higher cell proliferation at day 9, and four times higher alkaline phosphatase activity compared with untreated βTCP granules. Therefore, we concluded that regulation of surface morphology by a series of heat treatments is useful for improving cellular response to βTCP bone grafting materials.