Evidence from knockout mice for distinct implications of neuropeptide-Y Y2 and Y4 receptors in the circadian control of locomotion, exploration, water and food intake
Evidence from knockout mice for distinct implications of neuropeptide-Y Y2 and Y4 receptors in the circadian control of locomotion, exploration, water and food intake
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DOI：
10.1016/j.npep.2009.08.007
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发表时间：
2009-12-01
期刊：
影响因子：
2.9
通讯作者：
Holzer, P.
中科院分区：
文献类型：
--
作者：
Edelsbrunner, M. E.;Painsipp, E.;Holzer, P.
神经肽Y(NPY)家族的成员通过Y2和/或Y4受体作用，被认为参与控制摄食行为和能量平衡。由于这些过程在白天和晚上不同，我们探索了Y2(Y2-/-)或Y4(Y4-/-)受体基因突变小鼠的运动、探索和进食行为的昼夜模式。为此，使用了LabMaster系统，并对其用于分析由基因敲除引起的昼夜活动和摄取变化的效用进行了评估。雌性动物平均年龄27周，被单独饲养在装有水和食物摄入量传感器和两个红外线框的笼子中，用于记录走动和在12h光暗循环下饲养4d。与WT小鼠相比，Y2-/-小鼠的白天活动、探索、饮水和摄食减少，夜间活动增加。相比之下，Y4-/-小鼠在光相期间活动更多，但吃喝更少，而在暗期它们吃得更多，探索更少。Y2-/-和Y4-/-小鼠都比WT小鼠重。这些发现证明了Y2和Y4受体信号在平衡能量摄入和能量消耗的行为的昼夜控制中的不同作用。LabMaster系统可以灵敏地连续记录这些表型性状。(C)2009爱思唯尔有限公司，保留所有权利。
Members of the neuropeptide-Y (NPY) family acting via Y2 and/or Y4 receptors have been proposed to participate in the control of ingestive behaviour and energy homeostasis. Since these processes vary between day and night, we explored the circadian patterns of locomotor, exploratory and ingestive behaviour in mice with disrupted genes for Y2 (Y2-/-) or Y4 (Y4-/-) receptors. To this end, the LabMaster system was used and its utility for the analysis of changes in circadian activity and ingestion caused by gene knockout evaluated. Female animals, aged 27 weeks on average, were housed singly in cages fitted with sensors for water and food intake and two infrared frames for recording ambulation and rearing under a 12 h light/dark cycle for 4 days. Relative to WT animals, diurnal locomotion, exploration, drinking and feeding were reduced, whereas nocturnal locomotion was enhanced in Y2-/- mice. In contrast, Y4-/- mice moved more but ate and drank less during the photophase, while they ate more and explored less during the scotophase. Both Y2-/- and Y4-/- mice weighed more than WT mice. These findings attest to a differential role of Y2 and Y4 receptor signalling in the circadian control of behaviours that balance energy intake and energy expenditure. These phenotypic traits can be sensitively and continuously recorded by the LabMaster system. (C) 2009 Elsevier Ltd, All rights reserved.