A polycystic kidney-disease gene homologue required for male mating behaviour in C-elegans
A polycystic kidney-disease gene homologue required for male mating behaviour in C-elegans
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DOI：
10.1038/43916
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发表时间：
1999-09-23
期刊：
影响因子：
64.8
通讯作者：
Sternberg, PW
中科院分区：
文献类型：
--
作者：
Barr, MM;Sternberg, PW
秀丽隐杆线虫雄性的定型交配行为由几个子步骤组成：反应、支持、转向、外阴定位、骨针插入和精子转移。这种行为的复杂性反映在性二态解剖学和神经系统中（1）。通过细胞消融，行为功能已被分配给大多数男性特异性感觉神经元;例如，钩感觉神经元HOA和HOB是外阴位置特别需要的（2）。我们研究了C.雄性线虫控制交配行为。在这里，我们确定了一个基因，lov-1（外阴的位置），这是需要两个男性感官行为：反应和外阴位置。lov-1编码一种推定的膜蛋白，其具有粘蛋白样的富含丝氨酸-苏氨酸的氨基末端，随后是与人多囊蛋白同源的两个区段，人多囊蛋白是常染色体显性多囊肾疾病基因座PKD 1和PKD 2的产物（参考文献4）。LOV-1是最接近的C。PKD 1的elegans同源物。LOV-1在成年雄性中在鳐、钩和头的感觉神经元中表达，其分别介导对雌雄同体的反应、外阴定位和潜在的趋化性（2，5）。PKD-2、C. PKD 2的elegans同源物，定位于与LOV-1相同的神经元，表明它们在相同的通路中起作用。
The stereotyped mating behaviour of the Caenorhabditis elegans male is made up of several substeps: response, backing, turning, vulva location, spicule insertion and sperm transfer. The complexity of this behaviour is reflected in the sexually dimorphic anatomy and nervous system(1). Behavioural functions have been assigned to most of the male-specific sensory neurons by means of cell ablations; for example, the hook sensory neurons HOA and HOB are specifically required for vulva location(2). We have investigated how sensory perception of the hermaphrodite by the C. elegans male controls mating behaviours. Here we identify a gene, lov-1 (for location of vulva), that is required for two male sensory behaviours: response and vulva location. lov-1 encodes a putative membrane protein with a mucin-like, serine-threonine-rich amino terminus' followed by two blocks of homology to human polycystins, products of the autosomal dominant polycystic kidney-disease loci PKD1 and PKD2 (ref 4). LOV-1 is the closest C. elegans homologue of PKD1. lov-1 is expressed in adult males in sensory neurons of the rays, hook and head, which mediate response, vulva location, and potentially chemotaxis to hermaphrodites, respectively(2,5). PKD-2, the C. elegans homologue of PKD2, is localized to the same neurons as LOV-1, suggesting that they function in the same pathway.