High-Throughput Metabolic Profiling of Soybean Leaves by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
High-Throughput Metabolic Profiling of Soybean Leaves by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
复制标题
傅立叶变换离子回旋共振质谱法研究大豆叶片的高通量代谢。
DOI：
10.1021/acs.analchem.5b03340
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发表时间：
2016-01-19
影响因子：
7.4
通讯作者：
Wood TD
中科院分区：
文献类型：
--
作者：
Yilmaz A;Rudolph HL;Hurst JJ;Wood TD
植物代谢组学作为一个相对较新的研究领域，在过去的几年里得到了越来越多的关注，并已被应用于回答生物学问题，通过大规模的定性和定量分析的植物代谢组。质谱法（MS）提供的灵敏度和选择性相结合，可一次性测量许多代谢物，使其成为代谢组学中不可或缺的平台。在这方面，傅里叶变换离子回旋共振（FTICR）具有同时提供高质量分辨率和质量准确度的独特优势，使其成为研究复杂混合物（如植物提取物）的理想选择。在这里，我们优化大豆叶提取方法兼容高通量可重复MS为基础的代谢组学。此外，基质辅助激光解吸电离（MALDI）和直接激光解吸电离大豆叶片的代谢产物谱进行了比较。与电喷雾（ESI）-FTICR相结合的提取方法得到了叶绿素及其相关代谢产物的显着减少的支持，因为生长季节从仲夏到秋收日。据我们所知，第一次，ESI-FTICR MS和MALDI-FTICR MS的使用是以互补的方式描述的，目的是在生长季节的不同时间点收集的植物叶片的代谢谱。
As a relatively recent research field, plant metabolomics has gained increasing interest in the past few years and has been applied to answer biological questions through large-scale qualitative and quantitative analyses of the plant metabolome. The combination of sensitivity and selectivity offered by mass spectrometry (MS) for measurement of many metabolites in a single shot makes it an indispensable platform in metabolomics. In this regard, Fourier-transform ion cyclotron resonance (FTICR) has the unique advantage of delivering high mass resolving power and mass accuracy simultaneously, making it ideal for the study of complex mixtures such as plant extracts. Here we optimize soybean leaf extraction methods compatible with high-throughput reproducible MS-based metabolomics. In addition, matrix-assisted laser desorption ionization (MALDI) and direct LDI of soybean leaves are compared for metabolite profiling. The extraction method combined with electrospray (ESI)-FTICR is supported by the significant reduction of chlorophyll and its related metabolites as the growing season moves from midsummer to the autumn harvest day. To our knowledge for the first time, the use of ESI-FTICR MS and MALDI-FTICR MS is described in a complementary manner with the aim of metabolic profiling of plant leaves that have been collected at different time points during the growing season.