10-NM FILAMENTS IN NORMAL AND TRANSFORMED-CELLS
10-NM FILAMENTS IN NORMAL AND TRANSFORMED-CELLS
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DOI：
10.1016/0092-8674(78)90146-0
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发表时间：
1978-01-01
期刊：
影响因子：
64.5
通讯作者：
DESTREE, AT
中科院分区：
文献类型：
--
作者：
HYNES, RO;DESTREE, AT
针对来自培养的成纤维细胞（仓鼠 NIL8 细胞、小鼠 3T3 细胞）的电泳均质蛋白产生抗体，并显示该抗体针对 10 nm 丝。抗血清不染色微管或肌动蛋白微丝。研究了正常细胞在生长、铺展、运动、有丝分裂期间以及用秋水仙碱和细胞松弛素 B 处理后 10 nm 丝的分布。58,000 道尔顿亚基蛋白显然全部聚合在丝中，不溶于非离子洗涤剂。秋水仙碱处理会破坏微管，从而改变 10 nm 细丝的分布。 10 nm 细丝的组织在转化细胞中发生改变。
An antibody was raised against an electrophoretically homogeneous protein from cultured fibroblasts [hamster NIL8 cells, mouse 3T3 cells] and shown to be directed against 10 nm filaments. The antiserum did not stain microtubules or actin microfilaments. The distribution of 10 nm filaments in normal cells was studied during growth, spreading, locomotion, mitosis and after treatment with colchicine and cytochalasin B. The 58,000 dalton subunit protein is apparently all polymerized in the filaments which are insoluble in nonionic detergent. The distribution of 10 nm filaments is altered by colchicine treatments which disrupt microtubules. The organization of 10 nm filaments is altered in transformed cells.