REGULATION OF PYRIMIDINE BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
REGULATION OF PYRIMIDINE BIOSYNTHESIS IN SACCHAROMYCES CEREVISIAE
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DOI：
10.1128/jb.95.3.824-832.1968
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发表时间：
1968-01-01
影响因子：
3.2
通讯作者：
LACROUTE, F
中科院分区：
文献类型：
--
作者：
LACROUTE, F
酵母中的嘧啶途径的生化步骤被发现与细菌中的相同，除了一个步骤外，所有的步骤都得到了表征。前两种酶，氨基甲酰磷酸合成酶和天冬氨酸氨基转移酶的活性同时受到反馈抑制和抑制的控制。此外，这些酶由相同的遗传区(URA-2)编码，似乎形成了一个单一的酶复合体。途径后面的酶是由中间产物以连续的方式诱导的，对嘧啶的抑制不敏感。相应的基因(URA-4、URA-1、URA-3)既不相互连锁，也不与氨基甲酰磷酸合成酶和天冬氨酸氨基转移酶基因URA-2连锁。发现同时失去尿苷三磷酸对氨基甲酰磷酸合成酶和天冬氨酸氨甲酰基酶的反馈抑制的突变体被定位在基因URA-2中。
Biochemical steps of the pyrimidine pathway were found to be the same in yeast as in bacteria, and all except one step have been characterized. The activities of the first 2 enzymes, carbamoyl phosphate synthetase and aspartic transcarbamylase, are simultaneously controlled by feedback inhibition and repression. Moreover, these enzymes are coded by the same genetic region (ura-2) and seem to form a single enzymatic complex. The enzymes that follow later in the pathway are induced in a sequential way by the intermediary products and are insensitive to pyrimidine repression. The corresponding genes (ura-4, ura-1, ura-3) are not linked to each other or to ura-2, the gene for carbamoyl phosphate synthetase and aspartic transcarbamylase. Mutants that have simultaneously lost feedback inhibition by uridine triphosphate for carbamoyl phosphate synthetase and for aspartic transcarbamylase were found and mapped in the gene ura-2.