Human squamous cell carcinoma. Establishment and characterization of new permanent cell lines.
Human squamous cell carcinoma. Establishment and characterization of new permanent cell lines.
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DOI：
10.1001/archotol.1981.00790470051012
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发表时间：
1981-11
影响因子：
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通讯作者：
C. Krause;T. Carey;R. Ott;Charles G. Hurbis;K. Mcclatchey;J. Regezi
中科院分区：
文献类型：
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作者：
C. Krause;T. Carey;R. Ott;Charles G. Hurbis;K. Mcclatchey;J. Regezi
鳞状细胞癌是最常见的人类癌症，但由于它是由培养的细胞系代表性差，鲜为人知的是，这种肿瘤的特征细胞生物学和细胞表面抗原表型。为了开发一个可持续利用的鳞状细胞癌来源，用于重复和可重复的血清学分析，并更好地了解其生物学特性，采用组织培养方法和裸鼠建立新的鳞状细胞癌细胞系。特殊培养基、几种来源的血清补充剂和处理新鲜组织标本的方法都被作为提高肿瘤细胞系存活率的手段进行了检查。建立了几个新的细胞系。鳞状细胞起源的特征，如微绒毛，桥粒，张力细丝，鳞状细胞分化抗原（天疱疮抗原），被发现。
Squamous cell carcinoma is the most common of human cancers, and yet because it is poorly represented by cultured cell lines, little is known about the characteristic cell biology and the cell-surface antigenic phenotypes of such tumors. To develop a continuously available source of squamous cell carcinoma for repeated and reproducible serologic analysis and for better understanding of its biologic characteristics, tissue culture methods and nude mice were used to establish new cell lines of squamous carcinoma. Special media, serum supplements from several sources, and methods of handling fresh tissue specimens were all examined as a means of improving the survival of tumor cell lines. Several new cell lines were established. Features characteristic of a squamous cell origin, eg, microvilli, desmosomes, tonofilaments, and the squamous cell differentiation antigen (pemphigus antigen), were found. The clinical course of disease in individual donor patients has been examined.