HUMAN-BRAIN GLYCOGEN-PHOSPHORYLASE - CHARACTERIZATION OF FETAL CDNA AND GENOMIC SEQUENCES
HUMAN-BRAIN GLYCOGEN-PHOSPHORYLASE - CHARACTERIZATION OF FETAL CDNA AND GENOMIC SEQUENCES
复制标题
DOI：
10.1016/0169-328x(89)90052-1
复制
发表时间：
1989-11-01
期刊：
影响因子：
--
通讯作者：
GORIN, FA
中科院分区：
文献类型：
--
作者：
GELINAS, RP;FROMAN, BE;GORIN, FA
糖原磷酸化酶（α- 1，4-D-葡聚糖：正磷酸D-葡糖基转移酶，EC 2.4.1.1）是催化糖原降解的速率决定酶。人类大脑已经被证明以前表达的基因的肝脏和肌肉的糖原磷酸化酶同工酶。本文报道了人胎脑糖原磷酸化酶同工酶的cDNA和基因组DNA的分离和鉴定。对应于该同工酶的转录本存在于成人和胎儿脑中，并且在其他人胎儿组织中以低水平存在。由该cDNA和基因编码的蛋白质的预测C-末端序列不同于由从人星形细胞瘤细胞系分离的磷酸化酶cDNA编码的序列。
Glycogen phosphorylase (.alpha.-1,4-D-glucan:orthophosphate D-glucosyltransferase, EC 2.4.1.1) is the rate-determining enzyme catalyzing glycogen degradation. Human brain has been demonstrated previously to express genes of both the liver and muscle isozymes of glycogen phosphorylase. In this report, a human fetal brain cDNA and genomic DNA corresponding to the brain isozyme of glycogen phosphorylase were isolated and characterized. Transcripts corresponding to this isozyme are present in human adult and fetal brain, and at low levels in other human fetal tissues. The predicted C-terminal sequence of the protein encoded by this cDNA and gene differ from that encoded by a phosphorylase cDNA isolated from a human astrocytoma cell line.