Influence of global sperm DNA methylation on IVF results
Influence of global sperm DNA methylation on IVF results
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DOI：
10.1093/humrep/deh684
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发表时间：
2005-03-01
影响因子：
6.1
通讯作者：
Guérin, JF
中科院分区：
文献类型：
--
作者：
Benchaib, M;Braun, V;Guérin, JF
背景技术背景：在男性不育症的情况下，精子特征的常规分析是辅助生殖技术中分割率和胚胎发育的不良预测因素。据推测，表观遗传因素可能会影响胚胎的质量。这项工作的目的是确定精子DNA甲基化水平与受精率和妊娠率之间的关系，根据辅助生殖技术进行。方法：采用前瞻性研究。从接受辅助生殖手术的男性（n=63）中获得射精。5-甲基胞嘧啶用多克隆抗体进行免疫染色，并通过异硫氰酸荧光素显示。然后通过流式细胞术定量DNA甲基化水平。研究结果：进行了63个常规IVF周期，回收了760个卵母细胞，平均获得8.1+/-4.8个胚胎，移植了2.4个胚胎。受精率和优质胚胎率与DNA甲基化水平均无相关性（分别为r=-0.1和r=-0.08;均不显著）。当精子DNA甲基化>555任意单位时，妊娠率为33.3%，而低甲基化组为8.3%。
BACKGROUND: In cases of male infertility, routine analysis for sperm characteristics is a poor predictive factor for the segmentation rate and embryo development in assisted reproductive technologies. It is assumed that epigenetic factors could have an influence on the embryo's quality. The aim of this work was to determine the relationship between sperm DNA methylation level and fertilization and pregnancy rates according to the assisted reproduction technique performed. METHODS: A prospective study was undertaken. Ejaculates were obtained from men (n=63) undergoing an assisted reproduction procedure. 5-Methylcytosine was immunostained with a polyclonal antibody and revealed by fluorescein isothiocyanate. The DNA methylation level was then quantified by flow cytometry. RESULTS: Sixty-three conventional IVF cycles were performed, 760 oocytes were retrieved, an average of 8.1+/-4.8 embryos was obtained, and 2.4 embryos were transferred. Neither the fertilization rate nor the rate of good quality embryos was correlated with the DNA methylation level (r=-0.1 and r=-0.08 respectively; not significant). When sperm DNA methylation was >555 arbitrary units, the pregnancy rate was 33.3% compared with 8.3% in the lower (