Saliva from cheese bite yields DNA profile of burglar: a case report
Saliva from cheese bite yields DNA profile of burglar: a case report
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咬奶酪后的唾液可测出窃贼的 DNA 谱：病例报告
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
Dean P. Hildebrand
中科院分区：
文献类型：
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作者：
David Sweet;Dean P. Hildebrand
在犯罪现场发现的一块黄色奶酪上发现了高质量咬痕形式的抽象物证。这些奶酪在回收后被警方冷冻了10天，然后提交给实验室进行检测。采用双拭子技术采集DNA样本。获得了嫌疑人的血液样本。通过对10个STR基因座(Profiler Plus，Perkin Elmer应用生物系统公司)进行基于PCR的DNA分型，可以确定奶酪中的DNA来自嫌疑人。这一案例说明了a)始终将人类咬痕视为物理和生物证据，以及b)在任何可能存在微小唾液痕迹的情况下尝试DNA回收的重要性，即使在涉及富含细菌的食物的情况下也是如此。
Abstract Physical evidence in the form of a high quality bite mark was discovered on a piece of yellow cheese found at the scene of a crime. The cheese had been frozen by police for 10 days after recovery and before submission to the laboratory for testing. The double swab technique was used to collect DNA samples. A sample of the suspect’s blood was obtained. Using PCR-based DNA typing at ten STR loci, (Profiler Plus, Perkin Elmer-Applied Biosystems) it was determined that the DNA from the cheese originated from the suspect. This case illustrates the importance of a) always considering human bite marks as both physical and biological evidence, and b) attempting DNA recovery in any case in which minute traces of saliva may be present, even in situations involving bacteria-rich foods.