Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome
Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome
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DOI：
10.1002/pd.4307
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发表时间：
2014-04-01
影响因子：
3
通讯作者：
Masuzaki, Hideaki
中科院分区：
文献类型：
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作者：
Miura, Kiyonori;Higashijima, Ai;Masuzaki, Hideaki
目的本研究旨在鉴定一组主要是胎盘 (PP) mRNA，它们与后来发生的双胎输血综合征 (TTTS) 相关。方法首先，从我们之前报道的 50 个 PP mRNA 中，我们选择通常在母体血浆中可检测到的目标 mRNA。在随后并发 TTTS 的单绒毛膜双羊膜双胎 (MCDA-T) 妊娠 (n = 11) 和无并发症的 MCDA-T 妊娠 (n = 17) 中测量了这些 PP mRNA 的血浆浓度。最后，评估了血浆中PP mRNA的诊断价值。结果50个PP mRNA中，9个[人胎盘催乳素（hPL）；妊娠特异性糖蛋白 2 (PSG2)；人类妊娠特异性糖蛋白 3 (PSG3)；合胞素；合胞素2;视黄酸诱导14；含有解整合素和金属蛋白酶结构域的蛋白 12 (ADAM12)；绒毛膜糖蛋白激素、α多肽；选择[绒毛膜糖蛋白激素和β多肽]作为靶mRNA。在随后出现 TTTS 的 MCDA-T 孕妇中检测到母体血浆中 6 种 PP mRNA 的变化（hPL、PSG2 和 PSG3 增加，合胞素、合胞素2 和 ADAM12 减少）。最后，与单个 hPL mRNA 相比，mRNA 特征的 AUC 升高（hPL/PSG2：0.8717；hPL/PSG3：0.8449；hPL/ADAM12：0.8396）。结论母体血浆中多个无细胞 PP mRNA 的数量畸变先于临床明显的 TTTS 的出现。这表明胎盘的病理生理变化与 TTTS 的病态相关。 (c) 2013 约翰·威利父子有限公司
ObjectiveThis study aimed to identify a set of predominantly placental (PP) mRNAs, which are associated with later-developing twin-to-twin transfusion syndrome (TTTS).MethodFirst, out of 50 PP mRNAs we previously reported, we select target mRNAs that are ordinarily detectable in maternal plasma. Plasma concentrations of these PP mRNAs were measured in monochorionic diamniotic twin (MCDA-T) pregnancies complicated by TTTS later (n = 11) and in uncomplicated MCDA-T pregnancies (n = 17). Finally, the diagnostic values of the PP mRNAs in plasma were evaluated.ResultsFrom 50 PP mRNAs, nine [human placental lactogen (hPL); pregnancy-specific glycoproteins 2 (PSG2); human pregnancy-specific glycoproteins 3 (PSG3); syncytin; syncytin 2; retinoic acid-induced 14; A disintegrin and metalloproteinase domain-containing protein 12 (ADAM12); chorionic glycoprotein hormones, alpha polypeptide; and chorionic glycoprotein hormones, and beta polypeptide] were selected as target mRNAs. Changes in six PP mRNAs [increased hPL, PSG2, and PSG3 and decreased syncytin, syncytin2, and ADAM12] in maternal plasma were detected in MCDA-T pregnant women who subsequently developed TTTS. Finally, mRNA signatures gave elevated AUCs (hPL/PSG2: 0.8717; hPL/PSG3: 0.8449; hPL/ADAM12: 0.8396) compared with single hPL mRNA.ConclusionQuantitative aberration of plural cell-free PP mRNAs in maternal plasma precedes the appearance of clinically apparent TTTS. This suggests that pathophysiological changes in the placenta are associated with morbid conditions of TTTS. (c) 2013 John Wiley & Sons, Ltd.