The temporal changes of trabecular architecture in ovariectomized rats assessed by MicroCT
The temporal changes of trabecular architecture in ovariectomized rats assessed by MicroCT
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DOI：
10.1007/s001980170022
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发表时间：
2001-01-01
影响因子：
4
通讯作者：
Lane, NE
中科院分区：
文献类型：
--
作者：
Laib, A;Kumer, JL;Lane, NE
为了研究雌激素缺乏对小梁骨的短期和长期影响，采用显微计算机断层扫描（MicroCT）对去卵巢大鼠胫骨近端进行了三维测量。新的三维（3D）技术用于描述卵巢切除术（OVX）后0至110天的小梁结构。这些新方法不再假设骨的板或棒模型，而是通过在结构的3D表示中放置最大的球体来计算小梁的厚度、分离、数量和分布。采用结构模型指数（SMI）对骨模型类型进行量化。利用这些方法，我们发现OVX术后第一周骨小梁迅速丢失。第一周后，骨量进一步下降，尽管骨量丢失的速率较低。此外，ovx后7天内，模型类型从板状完全改变为杆状，12天后SMI非常稳定。小梁结构最初变薄后，切除小梁似乎会发生进一步的骨质流失，而小梁钢板厚度保持不变。网络的异质性可以通过。个体内标准，局部分离的偏差，表现为阶梯状进展，ovx后12至60天为平台期。本研究为三维显微ct评估卵巢切除术相关松质骨结构的变化提供了新的见解。此外，这些数据表明，ovx后模型从板状到棒状的快速变化可能会在使用基于模型的算法确定的参数中引入偏差，这些偏差可能会改变年龄相关或治疗变化的影响。
To study the short- and long-term effects of estrogen deficiency on trabecular bone, three-dimensional measurements of proximal, tibiae of ovariectomized rats were performed by micro-computed tomography (MicroCT). New three-dimensional (3D) techniques were employed to characterize the trabecular architecture from 0 to 110 days post-ovariectomy (OVX). These new methods no longer assume a plate or rod model of bone, but calculate trabecular thickness, separation, and number and their distribution by placing maximal spheres into the 3D representation of the structure. The model type of bone was quantified with the Structure Model Index (SMI). Utilizing these methods we found a rapid loss of trabecular bone in the first week after OVX. After the first week bone mass declined farther, although the rate of loss was lower. In addition there was a complete change in model type from plate-like to, rod-like within 7 days post-OVX, and then a very constant SMI after 12 days. After an initial thinning of trabecular structure, farther bone loss seems to occur through removal of trabeculae, while the trabecular plate thickness remains constant. The heterogeneity of the network could be quantified by. intraindividual standard, deviation of local separations, which showed a stair-like progression, with a plateau between 12 and 60 days post-OVX. This study provides new insights into ovariectomy-related changes in cancellous bone structure evaluated by 3D MicroCT. In, addition, these data suggest that the rapid change of model from plate-like to, rod-like post-OVX may potentially introduce biases in the parameters that are determined using model-based algorithms, and these biases may modify the impact of age-related or therapeutic changes.