Ca2+ uptake by cardiac sarcoplasmic reticulum from patients with idiopathic dilated cardiomyopathy.
Ca2+ uptake by cardiac sarcoplasmic reticulum from patients with idiopathic dilated cardiomyopathy.
复制标题
特发性扩张型心肌病患者心脏肌浆网对 Ca2 的摄取。
DOI：
10.1161/01.res.65.4.1141
复制
发表时间：
1989
影响因子：
20.1
通讯作者：
Krall,J
中科院分区：
文献类型：
--
作者：
Movsesian,MA;Bristow,MR;Krall,J
我们测定了6例正常人和9例IV型特发性扩张型心肌病患者的左室心肌肌浆网对钙离子的摄取。非心力衰竭心脏的钙摄取Vmax为593+/-82nmol/mg-min，K0.5为0.68+/-0.07微米，NHill为1.7+/-0.1。在切除的衰竭心脏中，相应的数值分别为593+/-36nmol/mg-min、0.63+/-0.03微米和1.6+/-0.1。未配型供者左室心肌粗肌膜β受体密度为110.0+/-15.3fmol/mg，切除衰竭心脏为52.1+/-4.5fmol/mg。这些结果表明，人类特发性扩张型心肌病的异常钙处理不是肌浆网对钙摄取的任何内在改变的结果。
We measured Ca2+ uptake by sarcoplasmic reticulum prepared from left ventricular myocardium obtained from six nonfailing human hearts and nine excised hearts from patients with class IV idiopathic dilated cardiomyopathy. Ca2+ uptake had a Vmax of 593 +/- 82 nmol/mg-min, a K0.5 of 0.68 +/- 0.07 microM, and an nHill of 1.7 +/- 0.1 in the nonfailing hearts. The corresponding values in the excised failing hearts were 593 +/- 36 nmol/mg-min, 0.63 +/- 0.03 microM, and 1.6 +/- 0.1. The beta-receptor density in crude sarcolemma prepared from left ventricular myocardium was 110.0 +/- 15.3 fmol/mg in the unmatched donors and 52.1 +/- 4.5 fmol/mg in the excised failing hearts. These results suggest that abnormal Ca2+ handling in idiopathic dilated cardiomyopathy in humans is not the result of any intrinsic alteration of Ca2+ uptake by sarcoplasmic reticulum.