Expression and evolutionary analyses of three acetylcholinesterase genes (Mi-ace-1, Mi-ace-2, Mi-ace-3) in the root-knot nematode Meloidogyne incognita
Expression and evolutionary analyses of three acetylcholinesterase genes (Mi-ace-1, Mi-ace-2, Mi-ace-3) in the root-knot nematode Meloidogyne incognita
复制标题
根结线虫根结线虫中三个乙酰胆碱酯酶基因（Mi-ace-1、Mi-ace-2、Mi-ace-3）的表达和进化分析。
DOI：
10.1016/j.exppara.2017.01.008
复制
发表时间：
2017-05-01
影响因子：
2.1
通讯作者：
Sun, Xiaotang
中科院分区：
文献类型：
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作者：
Cui, Ruqiang;Zhang, Lei;Sun, Xiaotang
克隆了南方根结线虫（Meloidogyne incognita）乙酰胆碱酯酶（AChE）基因Mi-ace-3的全长cDNA。Mi-ace-3开放阅读框为1875 bp，编码624个氨基酸残基。AChE结构和功能所必需的关键残基是保守的。推导的Mi-ACE-3蛋白序列与狄茎线虫Dd-AChE-3蛋白序列有72%的氨基酸相似性。系统发育分析表明，Mi-ACE-3与其他4种线虫的AChE形成了一个聚类。结果表明，本研究克隆的Mi-ace-3基因与秀丽隐杆线虫AChE同源，属于线虫ACE-3/4亚群。当Mi-ace-1、Mi-ace-2和Mi-ace-3同时被敲除时，转基因烟草根中的虫瘿数量显著减少，而当仅敲除这些基因中的一个或两个时，观察到很少或没有影响。这表明这三个基因的功能是多余的。(C)2017由Elsevier Inc.出版
The full cDNA of Mi-ace-3 encoding an acetylcholinesterase (AChE) in Meloidogyne incognita was cloned and characterized. Mi-ace-3 had an open reading frame of 1875 bp encoding 624 amino acid residues. Key residues essential to AChE structure and function were conserved. The deduced Mi-ACE-3 protein sequence had 72% amino acid similarity with that of Ditylenchus destructor Dd-AChE-3. Phylogenetic analyses using 41 AChEs from 24 species showed that Mi-ACE-3 formed a cluster with 4 other nematode AChEs. Our results revealed that the Mi-ace-3 cloned in this study, which is orthologous to Caenorhabditis elegans AChE, belongs to the nematode ACE-3/4 subgroup. There was a significant reduction in the number of galls in transgenic tobacco roots when Mi-ace-1, Mi-ace-2, and Mi-ace-3 were knocked down simultaneously, whereas little or no effect were observed when only one or two of these genes were knocked down. This is an indication that the functions of these three genes are redundant. (C) 2017 Published by Elsevier Inc.