PrimerCE: Designing Primers for Cloning and Gene Expression
PrimerCE: Designing Primers for Cloning and Gene Expression
复制标题
PrimerCE：设计用于克隆和基因表达的引物
DOI：
10.1007/s12033-010-9276-3
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发表时间：
2010-10-01
影响因子：
2.6
通讯作者：
Liu, Guozhen
中科院分区：
文献类型：
--
作者：
Cao, Yinghao;Sun, Jian;Liu, Guozhen
许多引物设计程序已开发用于不同的应用。然而，这些程序都不能用于设计用于以表达蛋白质为目的的基因克隆的引物。在这里，我们报告的PrimerCE的设计，它可以用来覆盖整个过程的基因克隆和表达。PrimerCE的主要功能包括限制性内切酶识别序列检测、开放阅读框验证、终止密码子检测、碱基调整、引物优化、序列拼接和蛋白质分析。除此之外，该程序可以根据用户的不同需求进行修改，例如可以整合新的载体序列和限制性内切酶识别序列。使用PrimerCE，可以在几分钟内设计出一对引物。该程序已被证明是有效的，在我们的高通量克隆和基因表达实验中的引物设计。该软件可在“http://tch.hebau.edu.cn/shengm/download/down.html“上免费获得。
A number of primer design programs have been developed for diverse applications. However, none of these programs can be used to design primers for gene cloning aimed at expressing protein. Here we report the design of PrimerCE, which can be used to cover the whole process of gene cloning and expression. The main features of PrimerCE include inspection of restriction enzyme recognition sequence, open reading frame verification, stop codon inspection, base adjustment, primer optimization, sequence assembly and protein analysis. In addition to this, the program can be modified based on the different needs of users, e.g. new vector sequence and restriction enzyme recognition sequences can be integrated. With the use of PrimerCE, a pair of primers can be designed within minutes. The program has been proven to be efficient in designing primers in our high throughput cloning and gene expression experiments. The software is freely available at "http://tch.hebau.edu.cn/shengm/download/down.html"