Global proteome turnover analyses of the Yeasts S. cerevisiae and S. pombe.
Global proteome turnover analyses of the Yeasts S. cerevisiae and S. pombe.
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DOI：
10.1016/j.celrep.2014.10.065
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发表时间：
2014-12-11
期刊：
影响因子：
8.8
通讯作者：
Walther TC
中科院分区：
文献类型：
--
作者：
Christiano R;Nagaraj N;Fröhlich F;Walther TC
细胞如何维持每种蛋白质的特定水平以及这种控制是否在进化上保守是关键问题。在这里，我们报告的蛋白质组范围内的稳态蛋白质周转率的测量进化上遥远，但生态相似的酵母，酿酒酵母和裂殖酵母粟酒裂殖酵母。我们发现大多数蛋白质的半衰期比目前想象的要长得多，并且在很大程度上取决于细胞分裂引起的蛋白质合成和稀释。然而，我们在两种酵母中检测到一个显著的蛋白质子集（~15%），这些蛋白质迅速翻转。此外，在4亿年的进化过程中，两种酵母之间的正构蛋白的相对丰度是高度保守的。相比之下，它们各自的更替率差别很大。我们的数据为研究常见酵母模型系统中的蛋白质降解提供了高置信度的资源。
How cells maintain specific levels of each protein and whether that control is evolutionarily conserved are key questions. Here, we report proteome-wide steady-state protein turnover rate measurements for the evolutionarily distant, but ecologically similar yeasts, Saccharomyces cerevisiae and Schizosaccharomyces pombe. We find that the half-lives of most proteins is much longer than currently thought and determined to a large degree by proteins synthesis and dilution due to cell division. However, we detect a significant subset of proteins (~15%) in both yeasts that are turned over rapidly. In addition, the relative abundances of orthologous proteins between the two yeasts are highly conserved across the 400 million years of evolution. In contrast, their respective turnover rates differ considerably. Our data provide a high-confidence resource for studying protein degradation in common yeast model systems.