Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung.
Synthesis and Application of Peptide-siRNA Nanoparticles from Disulfide-Constrained Cyclic Amphipathic Peptides for the Functional Delivery of Therapeutic Oligonucleotides to the Lung.
复制标题
二硫键限制的环状两亲性肽的肽-siRNA 纳米颗粒的合成和应用，用于将治疗性寡核苷酸功能性递送至肺。
DOI：
10.1007/978-1-0716-0928-6_4
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Nilsson,BradleyL
中科院分区：
文献类型：
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作者：
Welch,JadeJ;Dean,DavidA;Nilsson,BradleyL
RNAi治疗的潜力在很大程度上受到siRNA功能传递到细胞的内在挑战的阻碍。在这里，我们描述了由二硫键受限的两亲性多肽与siRNA络合而成的新型多肽-siRNA纳米颗粒的合成和表征方法，作为有希望的siRNA递送载体。我们还描述了将这些纳米颗粒应用于体外和体内将siRNA递送到肺细胞以功能击倒肺蛋白的方案。
The potential of RNAi therapies has been largely impeded by the inherent challenges in the functional delivery of siRNA to cells. Herein, we describe protocols for the synthesis and characterization of novel peptide–siRNA nanoparticles prepared from disulfide-constrained amphipathic peptides complexed with siRNA as promising siRNA delivery vectors. We also describe protocols for the application of these nanoparticles to the in vitro and in vivo delivery of siRNA to lung cells for the functional knockdown of lung proteins.