Extracellular HSP70 binding to surface receptors present on antigen presenting cells and endothelial/epithelial cells
Extracellular HSP70 binding to surface receptors present on antigen presenting cells and endothelial/epithelial cells
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DOI：
10.1016/j.febslet.2005.02.046
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发表时间：
2005-03-28
期刊：
影响因子：
3.5
通讯作者：
Calderwood, SK
中科院分区：
文献类型：
--
作者：
Thériault, JR;Mambula, SS;Calderwood, SK
已发现细胞外 HSP70 参与先天性和适应性免疫反应。然而，人们对介导这一过程的分子机制知之甚少。先前的报告表明 HSP70 通过大量表面受体与抗原呈递细胞 (APC) 相互作用。在这项研究中，我们检查了潜在 HSP70 受体的相对结合，发现与 LOX-1 具有高亲和力结合，但不与其他在 HSP70-APC 相互作用中起作用的结构结合，例如 LRP/CD91、CD40、TLR2、TLR4 或与 LOX-1 密切相关的另一个 c 型凝集素家族成员 (DC-SIGN)。除了 APC 之外，HSP70 还可以与非 APC 细胞系密切结合，尤其是来自上皮或内皮背景的细胞系。 (c) 2005 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Extracellular HSP70 has been found to participate in both innate and adaptive immune responses. However, little is known about the molecular mechanisms that mediate this process. Previous reports suggest that HSP70 interacts with antigen presenting cells (APC) through a plethora of surface receptors. In this study, we have examined the relative binding of potential HSP70 receptors and found high affinity binding to LOX-1 but not other structures with a role in HSP70-APC interactions such as LRP/CD91, CD40, TLR2, TLR4 or another c-type lectin family member (DC-SIGN) closely related to LOX-1. In addition to APC, HSP70 can avidly bind to non-APC cell lines, especially those from epithelial or endothelial background. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.