Cloning and characterization of a DCEIN2 gene responsive to ethylene and sucrose in cut flower carnation
Cloning and characterization of a DCEIN2 gene responsive to ethylene and sucrose in cut flower carnation
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切花康乃馨中对乙烯和蔗糖敏感的 DCEIN2 基因的克隆和表征
DOI：
10.1007/s11240-010-9886-2
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发表时间：
2011-06-01
影响因子：
3
通讯作者：
Yu, Yixun
中科院分区：
文献类型：
--
作者：
Fu, Zhaodi;Wang, Huinan;Yu, Yixun
康乃馨（Dianthus carpellusL.）是一种重要的观赏作物，是研究乙烯敏感花衰老的模式系统。乙烯不敏感基因EIN 2（ethylenesensitive2）是植物乙烯信号转导途径中的一个重要组成部分，但EIN 2基因对乙烯反应的转录调控机制尚不清楚。我们确定了一个cDNA克隆编码一个推定的EIN 2样蛋白（DCEIN 2）从总RNA分离衰老康乃馨花瓣使用逆转录PCR和cDNA末端的快速扩增程序。该cDNA含有一个3828 bp的开放阅读框，编码1275个氨基酸。北方杂交结果表明，外源乙烯和糖处理分别增强了花瓣和子房中DCEIN 2的表达，而硫代硫酸银则抑制了DCEIN 2的表达。在香石竹的营养组织中，DCEIN 2的mRNA在叶和茎中表达，而在根中未检测到。
Carnation (Dianthus caryophyllusL.) is an important ornamental crop and serves as a model system for investigating ethylene-sensitive flower senescence. EIN2 (ethylene insensitive 2) is a central component of the ethylene signal transduction pathway in plants, but the transcriptional regulation of the EIN2 gene in response to ethylene has not yet been elucidated. We identified a cDNA clone encoding a putative EIN2-like protein (DCEIN2) from total RNA isolated from senescing carnation petals using reverse transcription-PCR and rapid amplification of cDNA ends procedures. The cDNA contained an open reading frame of 3828 bp corresponding to 1275 amino acids. The northern blot results indicated thatDCEIN2expression in both the petals and ovaries was enhanced by treatment with exogenous ethylene and sugar, respectively, and was inhibited by silver thiosulfate. In the carnation vegetative tissues, mRNAs forDCEIN2were present in the leaves and stems, but they were not detected in the roots.