Beta-Adrenoceptor Agonists and Other cAMP Elevating Agents Suppress PAI-1 Production of Human Adipocytes in Primary Culture
Beta-Adrenoceptor Agonists and Other cAMP Elevating Agents Suppress PAI-1 Production of Human Adipocytes in Primary Culture
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β-肾上腺素受体激动剂和其他 cAMP 升高剂抑制原代培养中人脂肪细胞 PAI-1 的产生
DOI：
10.1055/s-2007-978678
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发表时间：
2000
影响因子：
2.2
通讯作者：
H. Hauner
中科院分区：
文献类型：
--
作者：
H. Gottschling;I. Aprath;T. Skurk;H. Hauner
最近的研究表明，儿茶酚胺有助于调节纤溶酶原激活物抑制剂-1（派-1）的表达，至少在内皮细胞中是这样。本研究的目的是研究儿茶酚胺对人脂肪细胞产生派-1的作用，特别是，以澄清哪些肾上腺素受体（AR）亚型参与。添加非选择性AR激动剂异丙肾上腺素导致肥胖受试者皮下和网膜贮库脂肪细胞中派-1 mRNA和蛋白释放的剂量和时间依赖性抑制。在瘦女性的皮下乳腺脂肪细胞中观察到类似程度的抑制。根据使用选择性激动剂的实验，这种作用主要通过β 2-肾上腺素受体介导。此外，除了cAMP升高剂，如二丁酰-cAMP，毛喉素和磷酸二酯酶抑制剂异丁基-甲基黄嘌呤和米力农导致不同程度的减少派-1。总之，本研究的结果支持这样的假设，即儿茶酚胺能够通过β-肾上腺素能受体下调人脂肪细胞中派-1的表达和分泌。
Recent studies showed that catecholamines contribute to the regulation of plasminogen activator inhibitor-1 (PAI-1) expression, at least in endothelial cells. Aim of this study was to examine the role of catecholamines on PAI-1 production by human adipocytes and, in particular, to clarify which adrenoceptor (AR) subtypes are involved. Addition of the unselective AR agonist isoproterenol led to a dose- and time-dependent suppression of PAI-1 mRNA and protein release in adipocytes from the subcutaneous and omental depot of obese subjects. A similar degree of suppression was observed in subcutaneous mammary adipocytes of lean women. This effect was mainly mediated via the beta2-adrenoceptor according to experiments using selective agonists. Moreover, addition of cAMP-elevating agents such as dibutyryl-cAMP, forskolin and the phosphodiesterase inhibitors isobutyl-methylxanthine and milrinone resulted in a reduction of PAI-1 of varying degrees. In conclusion, the results of this study support the assumption that catecholamines are able to down-regulate PAI-1 expression and secretion in human adipocytes via beta-adrenergic receptors.
影响因子：
15.9
作者：
Samad, F;Yamamoto, K;Loskutoff, DJ
通讯作者：
Loskutoff, DJ
影响因子：
6.5
作者：
M. Lafontan;M. Berlan
通讯作者：
M. Lafontan;M. Berlan