Visualization of circular DNA molecules labeled with colloidal gold spheres using atomic force microscopy
Visualization of circular DNA molecules labeled with colloidal gold spheres using atomic force microscopy
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使用原子力显微镜观察胶体金球标记的环状 DNA 分子
DOI：
10.1116/1.578311
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
D. D. Larson
中科院分区：
文献类型：
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作者：
W. Shaiu;J. Vesenka;Daniel M. Jondle;E. Henderson;D. D. Larson
我们用原子力显微镜（AFM）对金标记的DNA分子进行了成像。环状质粒DNA在内部位置用生物素化dUTP标记。为了可视化，在AFM成像之前，将生物素化的DNA连接到链霉亲和素涂层的胶体金球（名义上直径5 nm）。在干燥空气和丙醇下均可获得标记DNA的再现图像。介绍了在两种条件下制备的DNA和胶体金的高度测量。即使在丙醇作用下，DNA -链亲和素胶体金络合物的稳定性也表明，这种标记方法可以用于绘制染色体DNA中感兴趣的区域。
We have imaged gold‐labeled DNA molecules with the atomic force microscope (AFM). Circular plasmid DNA was labeled at internal positions by nick‐translation using biotinylated dUTP. For visualization, the biotinylated DNA was linked to streptavidin‐coated colloidal gold spheres (nominally 5 nm diam) prior to AFM imaging. Reproducible images of the labeled DNA were obtained both in dry air and under propanol. Height measurements of the DNA and colloidal gold made under both conditions are presented. The stability of the DNA‐streptavidin colloidal gold complexes observed even under propanol suggests that this labeling procedure could be exploited to map regions of interest in chromosomal DNA.