Secondary structure of the 5' end of bacteriophage MS2 RNA Methoxyamine and kethoxal modification.
Secondary structure of the 5' end of bacteriophage MS2 RNA Methoxyamine and kethoxal modification.
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噬菌体 MS2 RNA 5 端的二级结构甲氧胺和酮醛修饰。
DOI：
10.1111/j.1432-1033.1979.tb04277.x
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
W. Fiers
中科院分区：
文献类型：
--
作者：
D. Iserentant;W. Fiers
为了改进噬菌体MS 2基因组5'端的二级结构模型，用T1 RNase或Cm-RNase部分消化32 P标记的MS 2 RNA，分离5'端片段，复性并用甲氧胺或酮醛处理。用T1 RNase消化得到的修饰RNA，并通过微型指纹图谱分离产物。甲氧胺诱导的修饰暴露的胞苷检测的差分迁移率的修饰的寡核苷酸，而酮乙双胍诱导的改变暴露的鸟苷进行监测，通过对T1核糖核酸酶消化的阻力。根据改进的病毒RNA 5'端二级结构模型讨论了修饰残基的位置。结构本身的序列保守性和生物功能进行了讨论。
To refine the secondary structure model of the 5' end of the bacteriophage MS2 genome, 32P-labeled MS2 RNA was partially digested with T1 RNase or with Cm-RNase and the 5'-end fragment was isolated, renatured and submitted to treatment with methoxyamine or kethoxal. The resulting modified RNA was digested with T1 RNase and the products were separated by minifingerprinting. Methoxyamine-induced modification of exposed cytidines was detected by differential mobility of modified oligonucleotides, while kethoxal-induced alteration of exposed guanosines was monitored by resistance to T1 ribonuclease digestion. The positions of the modified residues are discussed in terms of an improved secondary structure model proposed for the 5' end of the viral RNA. The structure itself is discussed in relation to sequence conservation and biological function.