Antibiotic resistance genes in lakes from middle and lower reaches of the Yangtze River, China: Effect of land use and sediment characteristics
Antibiotic resistance genes in lakes from middle and lower reaches of the Yangtze River, China: Effect of land use and sediment characteristics
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中国长江中下游湖泊抗生素抗性基因：土地利用和沉积物特征的影响
DOI：
10.1016/j.chemosphere.2017.03.041
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发表时间：
2017
期刊：
影响因子：
8.8
通讯作者：
Wang Jun
中科院分区：
文献类型：
--
作者：
Yang Yuyi;Liu Wenzhi;Xu Chen;Wei Buqing;Wang Jun
淡水湖泊易受人为影响，为抗生素抗性基因（ARGs）的积累和繁殖提供了理想的媒介。在过去的几十年里，土地开垦和城市化对中国长江中下游湖泊产生了严重的人为影响。在这项研究中，选择了该地区的15个湖泊，以了解ARGs的水平和变化。以不同土地利用类型比例为指标，展示了各湖泊周边的土地复垦和城市化情况。对于磺胺类药物抗性（sul）基因，sul 1在沉积物中的相对丰度最高，最高值为2.11 × 10−1copies/16 SrRNA copy。四环素耐药（泰特）基因的平均相对丰度以tetG最高（4.74 × 10−3copies/16 SrRNA copies），其次为tetB、tetA、tetQ和tetM。Ⅰ类整合子（intI 1）在sul 1和tetG的获得和传播中起重要作用。沉积物特性（水分，密度，总氮，总碳，铵，硝酸盐）被认为对ARG分布没有显着影响。太湖和阳澄湖的建设用地比例较高，表现出较高的土地利用率。
Freshwater lakes provided an ideal media for the accumulation and propagation of antibiotic resistance genes (ARGs), because they were susceptible to anthropogenic impacts. Land reclamation and urbanization exerted severe anthropogenic impacts on lakes from middle and lower reaches of the Yangtze River, China over the past decades. In this study, 15 lakes in the region were selected to understand the level and variability of ARGs. Proportion of different land use types was applied to display the land reclamation and urbanization around each lake. For sulfonamide resistance (sul) genes,sul1 had the highest relative abundance in sediments, with maximum 2.11 × 10−1copies/16SrRNA copy in Gehu Lake. For tetracycline resistance (tet) genes,tetG had the highest average value of relative abundance (4.74 × 10−3copies/16SrRNA copy), followed bytetB,tetA,tetQ andtetM. Class I integron (intI1) played an important role in acquisition and dissemination ofsul1 andtetG. Sediment characteristics (moisture, density, total nitrogen, total carbon, ammonium, and nitrate) were found to have no significant effect on ARG distribution. Taihu Lake and Yangcheng Lake which exhibited highsulandtetgenes had the high proportion of built-up land use.