Expression of interferon-γ in human adrenal gland and kidney tumours
Expression of interferon-γ in human adrenal gland and kidney tumours
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DOI：
10.1038/sj.bjc.6603870
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发表时间：
2007-07-31
影响因子：
8.8
通讯作者：
Wang, H.
中科院分区：
文献类型：
--
作者：
Li, Q.;Zhang, X-q;Wang, H.
It is known that interferon-gamma ( IFN-gamma) is produced by activated T and NK lymphoid cells, mononuclear cells, and macrophage and dendritic cells. Our previous studies have shown that IFN- gamma like immunoreactivity also appears in human adrenal cortical tumour and phaeochromocytoma. To investigate whether human tumour cells can produce IFN- gamma, we examined 429 biopsy specimens of 30 kinds of tumour and tumour- surrounding tissues in adrenal glands and in kidneys by using immunohistochemistry and in situ hybridisation. IFN-gamma immunoactivity was shown in 34.3% of the adrenal cortical adenomas, 50% of the adrenal cortical carcinomas, 26.7% of the phaeochromocytomas, 26.7% of the clear cell renal cell carcinomas ( RCCs), 22% of the adrenal cortexes and 40% of medullas adjacent to tumours. The positive samples and expression areas were well overlapped between the IFN- g mRNA and the immunohistochemistry staining. Western blot analysis has further confirmed the immunohistochemistry results by showing a distinct IFN-gamma band corresponding to 17.4 kDa in tissue extracts from adrenal cortical adenoma, phaeochromocytoma and clear cell RCCs. These results indicate that IFN- gamma is produced by some types of tumour cells, suggesting it may play a dual role in the development of these tumours.
