Pilot Study on Oral Health Status as Assessed by an Active Matrix Metalloproteinase-8 Chairside Mouthrinse Test in Adolescents
Pilot Study on Oral Health Status as Assessed by an Active Matrix Metalloproteinase-8 Chairside Mouthrinse Test in Adolescents
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DOI：
10.1902/jop.2015.150377
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发表时间：
2016-01-01
影响因子：
4.3
通讯作者：
Sorsa, Timo
中科院分区：
文献类型：
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作者：
Heikkinen, Anna Maria;Nwhator, Solomon O.;Sorsa, Timo
背景：基质金属蛋白酶-8是参与牙周炎的一种主要的破坏性胶原酶，可作为牙周炎的生物标志物。中性粒细胞胶原酶2(活性基质金属蛋白酶-8[a-基质金属蛋白酶-8])口腔液体免疫分析最近被证明是成人牙周炎的危险指标。这项研究的目的是调查基于aMMP-8免疫分析的口腔护理点试验是否可以在早期有病理发现的青少年中识别口腔炎症负担(牙周炎和龋齿)患者。方法：这项横断面研究在芬兰Kotka卫生中心进行。首先，对受试者(15至17岁的青少年，n=47)进行aMMP-8主席侧咀嚼试验，并根据5分钟内的颜色变化读取结果。结果：探诊出血的敏感度和特异度分别为71.8%和77.5%，探诊深度分别为4 mm、48.3%和100%(P<；0.001)，探诊深度分别为4 mm、63.6%和100%(P<；0.001)；Pd和gt；分别为4 mm、76.5%和96.7%(P<；0.01)。对于牙周炎(1个Pd和Gt；=4 mm的部位)，几乎没有假阳性结果被识别。免疫分析法检测>；=1龋损的敏感度为76.5%，特异度为96.7%(P<；0.01)。结论：aMMP8主席试验在5分钟内即可检测出早期牙周炎患者的口腔炎症负担。也可以检测到龋损，但效率较低。
Background: Matrix metalloproteinase (MMP)-8 is a major destructive collagenase involved in periodontitis and can be regarded as a periodontitis biomarker. A neutrophil collagenase 2 (active MMP-8 [aMMP-8]) oral fluid immunoassay has recently been demonstrated to be a periodontitis risk indicator among adults. The aim of this study is to investigate whether a point-of-care mouthrinse test based on an aMMP-8 immunoassay could identify patients with oral inflammatory burden (periodontitis and caries) among adolescents with early pathologic findings.Methods: This cross-sectional study was carried out at the Kotka Health Center, Finland. First, the aMMP-8 chairside mouthrinse test was performed on enrolled individuals (adolescents aged 15 to 17 years, n = 47), and the results were read based on a color change within 5 minutes. Then, full-mouth clinical parameters of oral health were assessed, including periodontal, oral mucosal, and caries status.Results: The sensitivity and specificity of the test for bleeding on probing were 71.8% and 77.5%, respectively (P = 0.05); for >= 1 site with probing depth (PD) >= 4 mm, 48.3% and 100% (P < 0.001); for >= 2 sites with PD >= 4 mm, 63.6% and 100% (P < 0.001); and for >2 sites with PD >= 4 mm, 76.5% and 96.7% (P < 0.01). Regarding periodontitis (>= 1 site with PD >= 4 mm), hardly any false-positive results were identified. The sensitivity of the immunoassay for >= 1 caries lesions was 76.5%, and the specificity was 96.7% (P < 0.01).Conclusions: In 5 minutes, the aMMP-8 chairside test showed promising results, recognizing oral inflammatory burden in adolescents with early initial signs of periodontitis. Caries lesions could also be detected, but less efficiently.