PATTERN-SPECIFIC EXPRESSION OF THE DROSOPHILA-DECAPENTAPLEGIC GENE IN IMAGINAL DISKS IS REGULATED BY 3' CIS-REGULATORY ELEMENTS
PATTERN-SPECIFIC EXPRESSION OF THE DROSOPHILA-DECAPENTAPLEGIC GENE IN IMAGINAL DISKS IS REGULATED BY 3' CIS-REGULATORY ELEMENTS
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DOI：
10.1101/gad.4.11.2011
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发表时间：
1990-11-01
影响因子：
10.5
通讯作者：
HOFFMANN, FM
中科院分区：
文献类型：
--
作者：
MASUCCI, JD;MILTENBERGER, RJ;HOFFMANN, FM
果蝇大部分成虫角质层上的结构模式是在幼虫成虫的成体表盘上确定的结构模式。果蝇生长因子同系物Decapentaplecic(DPP)被认为参与了想象盘中图案的形成，主要是沿着将成为成体附件近端到远端的轴线。我们报道，DPP在翅膀、腿和眼睛触角成像盘中的表达定位于沿假定的近轴到远轴的一条细胞带。DPP在想象盘中的表达模式和水平受到突变病变的影响，这些突变病变移除了3‘’顺式调节序列。我们证明，3‘’顺式调节区的一部分含有调控元件，足以激活在想象盘中正常表达DPP的细胞子集的基因表达，从而挽救DPP突变表型。我们认为，完整的DPP表达模式是由3‘’调控元件组成的阵列产生的，这些元件在特定的盘中和盘中的某些位置具有不同的效力。对激活这些元素的因素的识别应该提供关于位置信息如何在想象盘中编码的线索。
The pattern of structures on most of the adult cuticle of Drosophila is determined in the larval imaginal disks. The Drosophila growth factor homolog decapentaplegic (dpp) is believed to participate in pattern formation in imaginal disks, primarily along what will become the proximal-to-distal axis of adult appendages. We report that dpp expression in wing, leg, and eye-antennal imaginal disks is localized to a band of cells along the presumptive proximal-to-distal axis. The pattern and level of dpp expression in imaginal disks is affected by mutant lesions that remove 3'' cis-regulatory sequences. We demonstrate that one portion of the 3'' cis-regulatory region contains regulatory elements sufficient to activate gene expression in a subset of the cells that normally express dpp in the imaginal disks, allowing rescue of dpp mutant phenotypes. We propose that the complete dpp expression pattern is generated by an array of 3'' regulatory elements that differ in their potency in specific disks and in certain positions within a disk. The identification of the factors that activate these elements should provide clues as to how positional information is encoded in imaginal disks.