Plasticity of cell migration resulting from mechanochemical coupling
Plasticity of cell migration resulting from mechanochemical coupling
复制标题
DOI：
10.7554/elife.48478
复制
发表时间：
2019-10-18
期刊：
影响因子：
7.7
通讯作者：
Rappel, Wouter-Jan
中科院分区：
文献类型：
--
作者：
Cao, Yuansheng;Ghabache, Elisabeth;Rappel, Wouter-Jan
真核细胞可以使用不同的模式迁移，从变形虫样（其间充满肌动蛋白的突起来来去去）到角膜细胞样（其特征在于稳定的形态和持续运动）。细胞如何在这些模式之间切换尚不清楚，但信号事件波被认为在这些转变中发挥着重要作用。在这里，我们提出了一个基于弛豫振荡器的简单二元生化反应扩散模型，并将其与细胞变形力学模型相结合。不同的迁移模式，包括类变形虫和类角膜细胞，通过生化行波、细胞力学和形态之间的相互作用决定的转变自然出现。在使用盘基网柄菌细胞的实验中，通过系统地降低肌动蛋白网络的突出力，模型的预测得到了明确的验证。我们的结果表明将信号传导事件与细胞力学和形态学耦合的重要性，并且可能适用于多种细胞运动系统。
Eukaryotic cells can migrate using different modes, ranging from amoeboid-like, during which actin filled protrusions come and go, to keratocyte-like, characterized by a stable morphology and persistent motion. How cells can switch between these modes is not well understood but waves of signaling events are thought to play an important role in these transitions. Here we present a simple two-component biochemical reaction-diffusion model based on relaxation oscillators and couple this to a model for the mechanics of cell deformations. Different migration modes, including amoeboid-like and keratocyte-like, naturally emerge through transitions determined by interactions between biochemical traveling waves, cell mechanics and morphology. The model predictions are explicitly verified by systematically reducing the protrusive force of the actin network in experiments using Dictyostelium discoideum cells. Our results indicate the importance of coupling signaling events to cell mechanics and morphology and may be applicable in a wide variety of cell motility systems.