Cell surface display of organophosphorus hydrolase in Pseudomonas putida using an ice-nucleation protein anchor
Cell surface display of organophosphorus hydrolase in Pseudomonas putida using an ice-nucleation protein anchor
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DOI：
10.1021/bp0340640
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发表时间：
2003-09-01
影响因子：
2.9
通讯作者：
Chen, W
中科院分区：
文献类型：
--
作者：
Shimazu, M;Nguyen, A;Chen, W
利用假单胞菌冰核蛋白（INP）的表面锚系统，将有机磷水解酶（OPH）定位于恶臭假单胞菌KT 2440的表面。携带编码INP-OPH融合体的穿梭载体pPNCO 33的细胞能够将OPH靶向到细胞表面上，如通过全细胞ELISA所证明的。恶臭假单胞菌KT 2440的全细胞活性显示比先前在大肠杆菌中表达相同融合蛋白的那些高10倍。在一个强大的和环境友好的恶臭假单胞菌KT 2440的表面上表达酶的能力，应该开辟了新的途径，为广泛的应用，如原位生物修复。
A surface anchor system derived from the ice-nucleation protein (INP) from Pseudomonas syringe was used to localize organophosphorus hydrolase (OPH) onto the surface of Pseudomonas putida KT2440. Cells harboring the shuttle vector pPNCO33 coding for the INP-OPH fusion were capable of targeting OPH onto the cell surface as demonstrated by whole cell ELISA. The whole cell activity of P. putida KT2440 was shown to be 10 times higher than those of previous efforts expressing the same fusion protein in Escherichia coli. The capability of expressing enzymes on the surface of a robust and environmentally benign P. putida KT2440 should open up new avenues for a wide range of applications such as in situ bioremediation.