MRT, Functioning with NURF Complex, Regulates Lipid Droplet Size.
MRT, Functioning with NURF Complex, Regulates Lipid Droplet Size.
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DOI：
10.1016/j.celrep.2018.08.026
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发表时间：
2018-09
期刊：
影响因子：
8.8
通讯作者：
Yan Yao;Xia Li;Wei Wang;Zhonghua Liu;Jianming Chen;Mei Ding;Xun Huang
中科院分区：
文献类型：
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作者：
Yan Yao;Xia Li;Wei Wang;Zhonghua Liu;Jianming Chen;Mei Ding;Xun Huang
脂滴（LD）是储存中性脂质的高度动态的细胞器。通过果蝇幼虫脂肪体中的基因过表达筛选，我们发现 MRT（一种含有 Myb/转换缺陷蛋白 3 (Swi3)、适配器 2 (Ada2)、核受体辅阻遏物 (N-CoR)、转录因子 (TF)IIIB (SANT) 样 DNA 结合结构域的蛋白）可调节 LD 大小和脂质储存。 MRT 直接与 PZG 和 NURF 染色质重塑复合体成分相互作用，并且在功能上依赖于 PZG 和 NURF 染色质重塑复合体成分。 MRT 与 plin1 的启动子（编码 LD 驻留蛋白 perilipin 的基因）结合，并抑制 plin1 的转录。体外 LD 聚结试验表明，mrt 过度表达或 plin1 功能丧失会促进 LD 聚结。我们的研究结果表明，MRT 与染色质重塑因子一起发挥作用，调节 LD 大小，可能是通过 plin1 的转录抑制实现的。
Lipid droplets (LDs) are highly dynamic organelles that store neutral lipids. Through a gene overexpression screen in theDrosophilalarval fat body, we have identified that MRT, an Myb/switching-defective protein 3 (Swi3), Adaptor 2 (Ada2), Nuclear receptor co-repressor (N-CoR), Transcription factor (TF)IIIB (SANT)-like DNA-binding domain-containing protein, regulates LD size and lipid storage. MRT directly interacts with, and is functionally dependent on, the PZG and NURF chromatin-remodeling complex components. MRT binds to the promoter ofplin1, the gene encoding the LD-resident protein perilipin, and inhibits the transcription ofplin1.In vitroLD coalescence assays suggest thatmrtoverexpression or loss ofplin1function facilitates LD coalescence. Our findings suggest that MRT functions together with chromatin-remodeling factors to regulate LD size, likely through the transcriptional repression ofplin1.