Cis1/Atg31 is required for autophagosome formation in Saccharomyces cereviside
Cis1/Atg31 is required for autophagosome formation in Saccharomyces cereviside
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DOI：
10.1016/j.bbrc.2007.02.150
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发表时间：
2007-05-04
影响因子：
3.1
通讯作者：
Ohsumi, Yoshinori
中科院分区：
文献类型：
--
作者：
Kabeya, Yukiko;Kawarnata, Tomoko;Ohsumi, Yoshinori
自噬是真核细胞的溶酶体/空泡中胞质物质的大量降解。在酿酒酵母中，已知有17种Atg蛋白参与自噬体的形成。全基因组分析显示Atgl 7与许多蛋白质相互作用。对这些相互作用蛋白的进一步研究可能会为自噬过程中的膜动力学提供进一步的见解。在这里，我们确定Cisl/Atg 3l作为一种蛋白质，表现出类似的表型Atg 17。在饥饿条件下，ATG 31无效细胞在自噬中有缺陷并且失去活力。Atg 3l定位于前-A utophago索马I结构（PAS）依赖于Atgl 7。免疫共沉淀实验表明Atg 3l与Atg 17相互作用。总之，Atg 31是一种新的蛋白质，与Atgl 7一致，是适当的自噬体形成所必需的。(c)2007年爱思唯尔公司All rights reserved.
Autophagy is the bulk degradation of cytosolic materials in lysosomes/vacuoles of eukaryotic cells. In the yeast SaccharomYces cerevisiae, 17 Atg proteins are known to be involved in autophagosome formation. Genorne wide analyses have shown that Atgl7 interacts with numerous proteins. Further studies on these interacting proteins may provide further insights into membrane dynamics during autophagy. Here, we identify Cisl/Atg3l as a protein that exhibits similar phenotypes to Atg17. ATG31 null cells were defective in aUtophagy and lost viability under starvation conditions. Localization of Atg3l to p re-a utophago soma I structures (PAS) was dependent on Atgl7. Coirnmunoprecipitation experiments indicated that Atg3l interacts with Atgl7. Together, Atg31 is a novel protein that, in concert with Atgl7, is required for proper autophagosome formation. (c) 2007 Elsevier Inc. All rights reserved.