Characterization of Nipah virus from outbreaks in Bangladesh, 2008-2010.
Characterization of Nipah virus from outbreaks in Bangladesh, 2008-2010.
复制标题
DOI：
10.3201/eid1802.111492
复制
发表时间：
2012-02
影响因子：
11.8
通讯作者：
Rota PA
中科院分区：
文献类型：
--
作者：
Lo MK;Lowe L;Hummel KB;Sazzad HM;Gurley ES;Hossain MJ;Luby SP;Miller DM;Comer JA;Rollin PE;Bellini WJ;Rota PA
新的基因分型方案有助于病毒序列的分类。尼帕病毒（NiV）是一种高致病性副粘病毒，可导致人类致命性脑炎。1998-1999年，马来西亚和新加坡首次爆发了NiV感染;自2001年以来，孟加拉国在人类中发生了相对较小的零星疫情。我们对2008年来自2名患者的相同NiV分离株的完整基因组序列和2010年来自3名患者的咽拭子样本的部分基因组序列进行了表征，所有患者均来自孟加拉国。来自孟加拉国患者的所有序列组成了一个独特的遗传组。然而，从法里德布尔和Gopalganj地区的患者中检测到3个遗传上不同的序列，表明在短时间内（2010年1月至3月）局部地区存在多个共循环谱系。所有可用的NiV基因的开放阅读框架之间的序列比较使我们提出了一个标准化的协议NiV基因分型，这个protcol提供了一个简单而准确的方法来分类当前和未来的NiV序列。
New genotyping scheme facilitates classification of virus sequences. Nipah virus (NiV) is a highly pathogenic paramyxovirus that causes fatal encephalitis in humans. The initial outbreak of NiV infection occurred in Malaysia and Singapore in 1998–1999; relatively small, sporadic outbreaks among humans have occurred in Bangladesh since 2001. We characterized the complete genomic sequences of identical NiV isolates from 2 patients in 2008 and partial genomic sequences of throat swab samples from 3 patients in 2010, all from Bangladesh. All sequences from patients in Bangladesh comprised a distinct genetic group. However, the detection of 3 genetically distinct sequences from patients in the districts of Faridpur and Gopalganj indicated multiple co-circulating lineages in a localized region over a short time (January–March 2010). Sequence comparisons between the open reading frames of all available NiV genes led us to propose a standardized protocol for genotyping NiV; this protcol provides a simple and accurate way to classify current and future NiV sequences.