Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy.
Analysis of Fluorescent Reporter Activity in the Male Germline During Pollen Development by Confocal Microscopy.
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DOI：
10.1007/978-1-4939-7286-9_6
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发表时间：
2017-09
影响因子：
--
通讯作者：
D. Twell;L. Brownfield
中科院分区：
文献类型：
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作者：
D. Twell;L. Brownfield
开花植物的雄性生殖系在雄配子体的营养细胞（花粉）内发育。生殖系是通过小孢子的不对称分裂形成生殖细胞而建立的。生殖细胞的有丝分裂然后产生双受精所需的两个精细胞。这些细胞分化产生配子附着和融合所需的蛋白质。了解种系发育的一个重要方面是种系基因表达的表征。在这里，我们描述了使用荧光报告研究生殖系基因表达发育花粉，以评估表达的时间和特异性。
The male germline of flowering plants develops within the vegetative cell of the male gametophyte (pollen). The germline is established by asymmetric division of the microspore to form the generative cell. Mitotic division of the generative cell then produces the two sperm cells required for double fertilization. These differentiate to produce the proteins required for gamete attachment and fusion. An important aspect of understanding germline development is the characterization of germline gene expression. Here, we describe the use of a fluorescent reporter to study germline gene expression in developing pollen to assess the timing and specificity of expression.