Effects of rainfall events on the occurrence and detection efficiency of viruses in river water impacted by combined sewer overflows
Effects of rainfall events on the occurrence and detection efficiency of viruses in river water impacted by combined sewer overflows
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DOI：
10.1016/j.scitotenv.2013.08.093
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发表时间：
2014-01-15
影响因子：
9.8
通讯作者：
Furumai, Hiroaki
中科院分区：
文献类型：
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作者：
Hata, Akihiko;Katayama, Hiroyuki;Furumai, Hiroaki
降雨事件可通过合流溢流（CSOs）的间歇排放将包括人类肠道病毒在内的大量微生物污染物引入地表水。本研究旨在探讨降雨事件对受CSO影响的地表水病毒载量的影响，以及分子方法检测肠道病毒的可靠性。通过对病毒浓度、核酸提取和逆转录(RT)-定量PCR （qPCR）步骤的过程控制来评估样本中病毒检测的可靠性，从而准确估计病毒检测效率。降雨期间收集的河水样本中脊髓灰质炎病毒的回收率（10%）。log（10）转化的病毒浓度效率与悬浮固体浓度呈负相关（r(2) = 0.86），悬浮固体浓度在降雨事件中显著增加。用腺病毒5型和引物共享对照分别测定DNA提取和qPCR步骤的效率在干燥天气下较低。然而，有机水质参数与这两个步骤的效率之间没有明显的关系。观察到的本地肠道腺病毒、gii -诺如病毒、肠道病毒和爱知病毒的浓度在降雨期间增加，尽管病毒浓度效率被认为低于干燥天气。本研究强调了使用适当的过程控制来准确评估受废水排放、雨水和公民社会组织影响的自然水体中水传播肠道病毒浓度的重要性。(C) 2013 Elsevier B.V.版权所有
Rainfall events can introduce large amount of microbial contaminants including human enteric viruses into surface water by intermittent discharges from combined sewer overflows (CSOs). The present study aimed to investigate the effect of rainfall events on viral loads in surface waters impacted by CSO and the reliability of molecular methods for detection of enteric viruses. The reliability of virus detection in the samples was assessed by using process controls for virus concentration, nucleic acid extraction and reverse transcription (RT)-quantitative PCR (qPCR) steps, which allowed accurate estimation of virus detection efficiencies. Recovery efficiencies of poliovirus in river water samples collected during rainfall events (10%). The log(10)-transformed virus concentration efficiency was negatively correlated with suspended solid concentration (r(2) = 0.86) that increased significantly during rainfall events. Efficiencies of DNA extraction and qPCR steps determined with adenovirus type 5 and a primer sharing control, respectively, were lower in dry weather. However, no clear relationship was observed between organic water quality parameters and efficiencies of these two steps. Observed concentrations of indigenous enteric adenoviruses, GII-noroviruses, enteroviruses, and Aichi viruses increased during rainfall events even though the virus concentration efficiency was presumed to be lower than in dry weather. The present study highlights the importance of using appropriate process controls to evaluate accurately the concentration of water borne enteric viruses in natural waters impacted by wastewater discharge, stormwater, and CSOs. (C) 2013 Elsevier B.V. All rights reserved.