Oxidative stress in the Dahl hypertensive rat
Oxidative stress in the Dahl hypertensive rat
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DOI：
10.1161/01.hyp.30.6.1628
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发表时间：
1997-12-01
期刊：
影响因子：
8.3
通讯作者：
Schmid-Schönbein, GW
中科院分区：
文献类型：
--
作者：
Swei, A;Lacy, F;Schmid-Schönbein, GW
氧自由基的产生增加可能通过影响血管平滑肌收缩、血流阻力和器官损伤而在高血压中发挥作用。本研究的目的是确定氧自由基是否参与盐性高血压的发展。Dahl盐敏感（Dahl-S）和盐抗性（Dahl-R）大鼠喂食高盐（6.0%NaCl）或低盐（0.3%NaCl）饮食4周。高盐饮食导致Dahl-S动物出现严重高血压，对Dahl-R动物的血压没有影响。四硝基蓝四唑染料用于检测肠系膜微血管中的超氧自由基。光吸收测量结果显示，高血压Dahl-S动物的小动脉和小静脉内皮沿着染色增强，而血压正常的动物染色值显著降低。此外，克拉克电化学电极用于测量新鲜血浆中的过氧化氢水平。高血压Dahl-S动物的血浆过氧化氢浓度高于血压正常的对应动物（2.81+/-0.43 vs. 2.10+/-0.41 μ mol/L），而在高盐和低盐处理的Dahl-R动物之间没有检测到差异（1.70+/-0.35 vs. 1.56+/-0.51 μ mol/L）。所有组的血浆过氧化氢水平与平均动脉压相关（r= 0.77）。这些研究结果表明，高血压Dahl-S大鼠的微血管中的氧自由基的产生增强。
Enhanced production of oxygen free radicals may play a role in hypertension by affecting vascular smooth muscle contraction, resistance to blood flow, and organ damage. The aim of this study was to determine whether oxygen free radicals are involved in the development of salt-induced hypertension. Dahl salt-sensitive (Dahl-S) and salt-resistant (Dahl-R) rats were fed either a high salt (6.0% NaCl) or low salt (0.3% NaCl) diet for 4 weeks. The high salt diet caused the development of severe hypertension in Dahl-S animals and had no effect on blood pressure in Dahl-R animals. A tetranitroblue tetrazolium dye was used to detect superoxide radicals in microvessels of the mesentery. Light absorption measurements revealed enhanced staining along the endothelium of arterioles and venules in hypertensive Dahl-S animals, with significantly lower values in normotensive animals. in addition, a Clark electrochemical electrode was used to measure hydrogen peroxide levels in fresh plasma. Hypertensive Dahl-S animals had a higher plasma hydrogen peroxide concentration compared with their normotensive counterparts (2.81+/-0.43 versus 2.10+/-0.41 mu mol/L), while no difference was detected between high- and low salt-treated Dahl-R animals (1.70+/-0.35 versus 1.56+/-0.51 mu mol/L). The plasma hydrogen peroxide levels of all groups correlated with mean arterial pressure (r=.77). These findings demonstrate an enhanced production of oxygen free radicals in the microvasculature of hypertensive Dahl-S rats.