Effects of oligomycins on adenosine triphosphatase activity of mitochondria isolated from the yeasts Saccharomyces cerevisiae and Schwanniomyces castellii
Effects of oligomycins on adenosine triphosphatase activity of mitochondria isolated from the yeasts Saccharomyces cerevisiae and Schwanniomyces castellii
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DOI：
10.1016/j.bbrc.2004.03.185
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发表时间：
2004-05-21
影响因子：
3.1
通讯作者：
Claisse, ML
中科院分区：
文献类型：
--
作者：
Charton, C;Ulaszewski, S;Claisse, ML
从酿酒酵母（Saccharomycescerevisiae）和卡氏许旺酵母（Schwanniomycescastellii）中分离出具有呼吸控制功能的线粒体。网站的存在，我在施。castellii的ADP/O比值高于S.酿酒酵母，该位点不存在。S.酿酒比在Schw. castellii线粒体DR 12核突变增加了后者。然而，热刺激和寡霉素对线粒体F1-F0 ATP酶活性的抑制作用在所有三个测试菌株中是相似的。In S.酿酒酵母和Schw.在卡斯泰利野生型或突变型线粒体中，低浓度寡霉素对F1-F0 ATP酶活性的众所周知的抑制在接近60 μ g/ml的高抑制剂浓度下被消除，表明F1活性解偶联。在更高的寡霉素浓度的ATP酶活性的两个物种和突变体再次强烈抑制，这表明对酵母F1活性的抑制作用尚未检测到。(C)2004年爱思唯尔公司All rights reserved.
Functional mitochondria with respiratory control were isolated from the yeasts Saccharomyces cerevisiae and Schwanniomyces castellii. The presence of site I in Schw. castellii was indicated by higher ADP/O ratio than in S. cerevisiae where this site is absent. The ATPase V-max was higher in S. cerevisiae than in Schw. castellii mitochondria. The latter was increased by the DR12 nuclear mutation. Nevertheless, the stimulation by heat and the inhibition profile of oligomycins on mitochondrial F1-F0 ATPase activities were similar in all three tested strains. In S. cerevisiae and Schw. castelli wild type or mutant mitochondria, the well-known inhibition of F1-F0 ATPase activity by low concentrations of oligomycins is abolished at high inhibitor concentrations near 60 mug/ml suggesting uncoupling of F1 activity. At still higher oligomycin concentration the ATPase activity of both species and mutant is again strongly inhibited, suggesting an inhibitory effect on yeast F1 activity not detected so far. (C) 2004 Elsevier Inc. All rights reserved.