ALTERATION OF URACIL‐DNA GLYCOSYLASE ACTIVITY BY URACIL DIMERS IN DNA *
ALTERATION OF URACIL‐DNA GLYCOSYLASE ACTIVITY BY URACIL DIMERS IN DNA *
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DNA 中尿嘧啶二聚体对尿嘧啶 DNA 糖基化酶活性的改变 *
DOI：
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发表时间：
1981
影响因子：
3.3
通讯作者：
D. Hart
中科院分区：
文献类型：
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作者：
N. Duker;W. Davies;D. Hart
摘要-研究了紫外线照射对双链PBS-2噬菌体DNA（含有尿嘧啶代替胸腺嘧啶）的影响。在三重态敏化剂苯乙酮的存在下，用254 nm光或313 nm光照射，导致产生含尿嘧啶的嘧啶光二聚体。在这两种条件下也产生DNA链断裂。尿嘧啶-DNA糖基化酶释放游离尿嘧啶，但不从这种辐照的DNA中释放含尿嘧啶的二聚体。随着每个DNA分子的光二聚体数量增加，尿嘧啶从辐照的PBS-2 DNA中酶促释放的速率降低。
Abstract— The effects of UV irradiation on double‐stranded PBS‐2 phage DNA, which contains uracil in place of thymine, were studied. Irradiation by 254 nm light, or by 313 nm light in the presence of the triplet sensitizer acetophenone, resulted in production of uracil‐containing pyrimidine photodimers. DNA chain breaks were also produced under both conditions. Uracil‐DNA glycosylase released free uracil, but no uracil‐containing dimers from such irradiated DNA. The rate of enzymatic release of uracil from irradiated PBS‐2 DNA was decreased as the number of photodimers per DNA molecule was increased.